Comparative Proteomic Profile of the Human Umbilical Cord Blood Exosomes between Normal and Preeclampsia Pregnancies with High-Resolution Mass Spectrometry

被引：59

作者：

Jia, Ruizhe ^{[1
]}

Li, Jingyun ^{[2
]}

Rui, Can ^{[1
]}

Ji, Hui ^{[1
]}

Ding, Hongjuan ^{[1
]}

Lu, Yuanqing ^{[1
]}

De, Wei ^{[3
]}

Sun, Lizhou ^{[4
]}

机构：

[1] Nanjing Med Univ, Nanjing Matern & Child Hlth Care Hosp, Dept Obstet, Nanjing 210029, Jiangsu, Peoples R China

[2] Nanjing Med Univ, Nanjing Matern & Child Hlth Care Hosp, Dept Plast & Cosmet Surg, State Key Lab Reprod Med, Nanjing 210029, Jiangsu, Peoples R China

[3] Nanjing Med Univ, Nanjing 210029, Jiangsu, Peoples R China

[4] Nanjing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Nanjing 210029, Jiangsu, Peoples R China

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2015年 / 36卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Exosomes; Proteomic profile; Umbilical cord blood; Preeclampsia; High-resolution mass spectrometry; GROWTH RESTRICTION; PLACENTA; EXPRESSION; PROTEINS; WOMEN;

D O I：

10.1159/000430193

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Background/Aims: Exosomes are extracellular vesicles that are involved in several biological processes. The roles of proteins from human umbilical cord blood exosomes in the pathogenesis of preeclampsia remains poorly understood. Methods: In this study, we used high-resolution LC-MS/MS technologies to construct a comparative proteomic profiling of human umbilical cord blood exosomes between normal and preeclamptic pregnancies. Results: A total of 221 proteins were detected in human umbilical cord blood exosomes, with 14 upregulated and 15 downregulated proteins were definitively identified between preeclamptic and control pregnancies. Further bioinformatics analysis (Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis) indicated that these differentially expressed proteins correlate with enzyme regulator activity, binding, extracellular region, cell part, biological regulation, cellular process and complement and coagulation cascades occurring during pathological changes of preeclampsia. Conclusion: Our results show significantly altered expression profiles of proteins in human umbilical cord blood exosomes between normal and preeclampsia pregnancies. These proteins may be involved in the etiology of preeclampsia. Copyright (C) 2015 S. Karger AG, Basel

引用

页码：2299 / 2306

页数：8

共 32 条

[1]

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