Phosphoproteomic investigation of a solvent producing bacterium Clostridium acetobutylicum

被引:23
作者
Bai, Xue [1 ,2 ]
Ji, Zhihong [1 ,3 ]
机构
[1] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
[2] Chinese Acad Sci, Beijing Inst Genom, Beijing 100029, Peoples R China
[3] Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
关键词
Phosphoproteome; Mass spectrometry; Clostridium acetobutylicum; Biological solvent production; PROTEIN-PHOSPHORYLATION; ESCHERICHIA-COLI; SOLVENTOGENESIS; ACIDOGENESIS; ACETATE; EXPRESSION; BUTYRATE; ATCC-824; CULTURES; KINASE;
D O I
10.1007/s00253-012-4156-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In this study, we employed TiO2 enrichment and high accuracy liquid chromatography-mass spectrometry-mass spectrometry to identify the phosphoproteome of Clostridium acetobutyicum ATCC824 in acidogenesis and solventogenesis. As many as 82 phosphopeptides in 61 proteins, with 107 phosphorylated sites on serine, threonine, or tyrosine, were identified with high confidence. We detected 52 phosphopeptides from 44 proteins in acidogenesis and 70 phosphopeptides from 51 proteins in solventogenesis, respectively. Bioinformatic analysis revealed most of the phosphoproteins located in cytoplasm and participated in carbon metabolism. Based on comparison between the two stages, we found 27 stage-specific phosphorylated proteins (10 in acidogenesis and 17 in solventogenesis), some of which were solvent production-related enzymes and metabolic regulators, showed significantly different phosphorylated status. Further analysis indicated that protein phosphorylation could be involved in the shift of stages or in solvent production pathway directly. Comparison against several other organisms revealed the evolutionary diversity among them on phosphorylation level in spite of their high homology on protein sequence level.
引用
收藏
页码:201 / 211
页数:11
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