Simple and sensitive method for the determination of celecoxib in human serum by high-performance liquid chromatography with fluorescence detection

被引:36
作者
Schönberger, F [1 ]
Heinkele, G [1 ]
Mürdter, TE [1 ]
Brenner, S [1 ]
Klotz, U [1 ]
Hofmann, U [1 ]
机构
[1] Dr Margarete Fischer Bosch Inst Clin Pharmacol, D-70376 Stuttgart, Germany
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 768卷 / 02期
关键词
celecoxib;
D O I
10.1016/S1570-0232(01)00588-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple method is described for the determination of the cyclooxygenase-2 specific inhibitor celecoxib in human serum by HPLC using the demethylated analogue as internal standard. After protein precipitation with acetonitrile, samples were extracted with chloroform. Separation was achieved on a Prontosil C-18 AQ column (150X3 mm I.D., 3-mum particle size) at a flow-rate of 0.35 ml/min using water-acetonitrile (40:60, v/v) as the mobile phase. Using fluorescence detection with excitation at 240 nm and emission at 380 nm, the limit of quantification was 12.5 ng/ml for a sample size of 0.5 ml of serum. The assay was linear in the concentration range of 12.5-1500 ng/ml and showed good accuracy and reproducibility. At all concentrations intra- and inter-assay variabilities were below 11% with less than 9% error, The method was applied to the determination of celecoxib for pharmacokinetic studies in man. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:255 / 260
页数:6
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