Structure and function of plant protein-derived antihypertensive peptides

The main targets for antihypertensive peptides are renin and angiotensin converting enzyme (ACE). Plant protein-based inhibitory agents are first generated as enzymatic protein hydrolysates, which contain a pool of peptides with different sizes and effectiveness. Protein hydrolysate fractions that consist of mainly low molecular weight peptides (usually <3 kDa) are more effective inhibitors of renin and ACE than the bigger peptides. Purifications of protein hydrolysates have yielded several peptides that inhibit in vitro renin and ACE activities in addition to exerting in vivo blood pressure reductions. The structural basis for enhanced peptide activity varies but generally peptides that contain proline, branched-chain amino acids and aromatic amino acids have strong inhibitory activities against renin and ACE.