Immortalization of epithelial cells

被引：37

作者：

Hopfer, U

Jacobberger, JW

Gruenert, DC

Eckert, RL

Jat, PS

Whitsett, JA

机构：

[1] CASE WESTERN RESERVE UNIV, SCH MED, DEPT DERMATOL, CLEVELAND, OH 44106 USA

[2] CASE WESTERN RESERVE UNIV, SCH MED, DEPT GENET, CLEVELAND, OH 44106 USA

[3] CASE WESTERN RESERVE UNIV, SCH MED, DEPT BIOCHEM, CLEVELAND, OH 44106 USA

[4] CASE WESTERN RESERVE UNIV, SCH MED, CTR CANC, CLEVELAND, OH 44106 USA

[5] CHILDRENS HOSP, MED CTR, DIV PULM BIOL, CINCINNATI, OH 45229 USA

[6] UNIV CALIF SAN FRANCISCO, CARDIOVASC RES INST, DEPT LAB MED, GENE THERAPY CORE CTR, SAN FRANCISCO, CA 94143 USA

[7] UCL MED SCH BRANCH, LUDWIG INST CANC RES, LONDON W1P 8BT, ENGLAND

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1996年 / 270卷 / 01期

关键词：

simian virus 40; papillomavirus; large T antigen; Papovaviridae; H-2K(b)tsA58 transgenic mouse; lung; intestine; kidney; cervix; human; rat; mouse; rabbit; cystic fibrosis;

D O I：

10.1152/ajpcell.1996.270.1.C1

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The methodologies for isolating cell lines have become very powerful, particularly in terms of retaining differentiated features of the parent cells. Cell lines can be developed from primary or early passage cells as well as from transgenic animals that carry an immortalizing gene. Cell lines from epithelia have been selected for their polar orientation, tight junction formation, and expression of differentiated markers or functions. These cell lines provide useful models for studying cell biology of specific tissues, tumorigenicity, genetic abnormalities, or to help screen for effective methods of gene therapy.

引用

页码：C1 / C11

页数：11

共 93 条

[31] CULTURED HUMAN ECTOCERVICAL EPITHELIAL-CELL DIFFERENTIATION IS REGULATED BY THE COMBINED DIRECT ACTIONS OF SEX STEROIDS, GLUCOCORTICOIDS, AND RETINOIDS [J].