Comparison of the new Abbott Real Time CMV assay and the Abbott CMV PCR Kit for the quantitation of plasma cytomegalovirus DNAemia

被引:41
作者
Angeles Clari, Maria [1 ]
Bravo, Dayana [1 ]
Costa, Elisa [1 ]
Munoz-Cobo, Beatriz [1 ]
Solano, Carlos [2 ]
Jose Remigia, Maria [2 ]
Gimenez, Estela [1 ]
BenMarzouk-Hidalgo, Omar J. [3 ]
Perez-Romero, Pilar [3 ]
Navarro, David [1 ,4 ]
机构
[1] Univ Clin Hosp, Microbiol Serv, Valencia, Spain
[2] Univ Clin Hosp, Hematol & Med Oncol Serv, Valencia, Spain
[3] Univ Hosp Virgen del Rocio, Inst Biomed Sevilla IBiS, Unit Infect Dis Microbiol & Prevent Med, Seville, Spain
[4] Univ Valencia, Sch Med, Dept Microbiol, E-46003 Valencia, Spain
关键词
Cytomegalovirus; Real-time PCR; Plasma CMV DNAemia; Abbott PCR assays; CELL TRANSPLANT RECIPIENTS; DNA; QUANTIFICATION; INFECTION;
D O I
10.1016/j.diagmicrobio.2012.10.010
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
CMV DNA loads measured by the new Abbott RealTime CMV PCR were significantly higher than those quantitated by the Abbott CMV PCR kit (approximately 1 log(10)), and provided a better estimate of the actual CMV load present in plasma specimens as inferred by the use of the WHO standard. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码:207 / 209
页数:3
相关论文
共 9 条
[1]   STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF CLINICAL MEASUREMENT [J].
BLAND, JM ;
ALTMAN, DG .
LANCET, 1986, 1 (8476) :307-310
[2]   Human cytomegalovirus DNA in plasma and serum specimens of renal transplant recipients is highly fragmented [J].
Boom, R ;
Sol, CJA ;
Schuurman, T ;
van Breda, A ;
Weel, JFL ;
Beld, M ;
ten Berge, IJM ;
Wertheim-van Dillen, PME ;
de Jong, MD .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (11) :4105-4113
[3]   Comparative Evaluation of Three Automated Systems for DNA Extraction in Conjunction with Three Commercially Available Real-Time PCR Assays for Quantitation of Plasma Cytomegalovirus DNAemia in Allogeneic Stem Cell Transplant Recipients [J].
Bravo, Dayana ;
Angeles Clari, Maria ;
Costa, Elisa ;
Munoz-Cobo, Beatriz ;
Solano, Carlos ;
Jose Remigia, Maria ;
Navarro, David .
JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (08) :2899-2904
[4]   Evaluation of real-time PCR laboratory-developed tests using analyte-specific reagents for cytomegalovirus quantification [J].
Caliendo, Angela M. ;
Ingersoll, Jessica ;
Fox-Canale, Andrea M. ;
Pargman, Sabine ;
Bythwood, Tameka ;
Hayden, Mary K. ;
Bremer, James W. ;
Lurain, Nell S. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (06) :1723-1727
[5]  
Fryer JF., 2010, WHOBS102138
[6]   Quantification of DNA in plasma by an automated real-time PCR assay (cytomegalovirus PCR kit) for surveillance of active cytomegalovirus infection and guidance of preemptive therapy for allogeneic hematopoietic stem cell transplant recipients [J].
Gimeno, Concepcion ;
Solano, Carlos ;
Latorre, Jose C. ;
Hernandez-Boluda, Juan C. ;
Clari, Maria A. ;
Remigia, Maria J. ;
Furio, Santiago ;
Calabuig, Marisa ;
Tormo, Nuria ;
Navarro, David .
JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (10) :3311-3318
[7]   Differences in cytomegalovirus plasma viral loads measured in allogeneic hematopoietic stem cell transplant recipients using two commercial real-time PCR assays [J].
Gracia-Ahufinger, Irene ;
Tormo, Nuria ;
Espigado, Ildefonso ;
Solano, Carlos ;
Urbano-Ispizua, Alvaro ;
Clari, Maria A. ;
de la Cruz-Vicente, Fatima ;
Navarro, David ;
Perez-Romero, Pilar .
JOURNAL OF CLINICAL VIROLOGY, 2010, 48 (02) :142-146
[8]   Interlaboratory Comparison of Cytomegalovirus Viral Load Assays [J].
Pang, X. L. ;
Fox, J. D. ;
Fenton, J. M. ;
Miller, G. G. ;
Caliendo, A. M. ;
Preiksaitis, J. K. .
AMERICAN JOURNAL OF TRANSPLANTATION, 2009, 9 (02) :258-268
[9]   Clinical virology of cytomegalovirus infection following hematopoietic transplantation [J].
Solano, Carlos ;
Navarro, David .
FUTURE VIROLOGY, 2010, 5 (01) :111-124