Determination of ranitidine in urine by capillary electrophoresis-electrochemiluminescent detection

被引：49

作者：

Gao, Y

Tian, YL

Sun, XH

Yin, XB

Xiang, Q

Ma, G

Wang, EK ^{[1
]}

机构：

[1] Chinese Acad Sci, State Key Lab Electroanalyt Chem, Changchun Inst Appl Chem, Changchun, Peoples R China

[2] Tianjin Univ, Dept Chem, Tianjin 300072, Peoples R China

[3] Changchun Inst Technol, Dept Appl Chem, Changchun, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 832卷 / 02期

基金：

中国国家自然科学基金;

关键词：

capillary electrophoresis; electrochemiluminescence; ranitidine;

D O I：

10.1016/j.jchromb.2006.01.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The fast analysis of ranitidine is of clinical importance in understanding its efficiency and a patient's treatment history. In this paper, a novel determination method for ranitidine based on capillary electrophoresis-electrochemiluminescence detection is described. The conditions affecting separation and detection were investigated in detail. End-column detection of ranitidine in 5 mM Ru(bpy)(3)(2+) solution at applied voltage of 1.20 V was performed. Favorable ECL intensity with higher column efficiency was achieved by electrokinetic injection for 10 s at 10 kV. The R.S.D. values of ECL intensity and migration time were 6.38 and 1.84% for 10(-4) M and 6.01 and 0.60% for 10(-5) M, respectively. A detection limit of 7 x 10(-8) M (S/N = 3) was achieved. The proposed method was applied satisfactorily to the determination of ranitidine in urine in 6 min. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：236 / 240

页数：5

共 27 条

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