Essential roles of the κ light chain intronic enhancer and 3′ enhancer in κ rearrangement and demethylation

被引:90
作者
Inlay, M
Alt, FW
Baltimore, D
Xu, Y
机构
[1] Univ Calif San Diego, Div Biol, Mol Biol Sect, La Jolla, CA 92093 USA
[2] Harvard Univ, Sch Med, Howard Hughes Med Inst, Childrens Hosp,Ctr Blood Res, Boston, MA 02115 USA
[3] CALTECH, Div Biol, Pasadena, CA 91125 USA
关键词
D O I
10.1038/ni790
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The kappa intronic (MiE(kappa)) and 3' (3'E-kappa) enhancers are both quantitatively important to, but not essential for, immunoglobulin kappa rearrangement. To determine the functional redundancy between these two enhancers, B cells derived from mutant embryonic stem cells-in which both MiE(kappa) and 3'E-kappa were deleted on both kappa alleles-were analyzed for kappa rearrangement. Our findings indicate that these double-mutant B cells have essentially no kappa rearrangement but do rearrange and express lambda. Therefore, these two kappa enhancers share essential roles in activatingV(kappa)J(kappa) rearrangement. Our findings also indicate that the two kappa enhancers play overlapping and distinct roles in the demethylation of kappa in B cells.
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页码:463 / 468
页数:6
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