共 40 条
V(D)J recombination is not activated by demethylation of the kappa locus
被引:30
作者:

Cherry, SR
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机构: CALTECH, Pasadena, CA 91125 USA

Beard, C
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机构: CALTECH, Pasadena, CA 91125 USA

Jaenisch, R
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机构: CALTECH, Pasadena, CA 91125 USA

Baltimore, D
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机构:
CALTECH, Pasadena, CA 91125 USA CALTECH, Pasadena, CA 91125 USA
机构:
[1] CALTECH, Pasadena, CA 91125 USA
[2] MIT, Cambridge, MA 02139 USA
[3] Whitehead Inst, Cambridge Ctr 9, Cambridge, MA 02142 USA
来源:
关键词:
D O I:
10.1073/pnas.150218497
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
V(D)J recombination is thought to be regulated by changes in the accessibility of target sites, such as modulation of methylation. To test whether demethylation of the kappa locus can activate recombination, we generated two recombinationally active B cell lines in which the gene for maintenance of genomic DNA methylation, Dnmt1, was flanked with loxP sites. Transduction with a retrovirus expressing both the cre recombinase and green fluorescent protein allowed us to purify recombinationally active cells devoid of methylation. Loss of methylation of the kappa locus was not sufficient to activate recombination. although transcription was activated in one line. It appears that demethylation of the kappa locus is not the rate-limiting step for altering accessibility and thus regulated demethylation does not generate specificity of recombination.
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页码:8467 / 8472
页数:6
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