Use of sequence characterised amplified region and RAPD markers in the identification of the white truffle Tuber magnatum Pico

被引：20

作者：

Amicucci, A ^{[1
]}

Rossi, I ^{[1
]}

Potenza, L ^{[1
]}

Agostini, D ^{[1
]}

Stocchi, V ^{[1
]}

机构：

[1] UNIV URBINO,IST CHIM BIOL,I-61029 URBINO,PS,ITALY

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 03期

关键词：

D O I：

10.1023/A:1018493111804

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Isolates of white truffles were identified as Tuber magnatum Pico species using a pair of primers selected from a sequence characterised amplified region (SCAR) and a specific random amplified polymorphic DNA (RAPD) marker. The present study reveals that PCR-fragment-pattern polymorphisms, the construction of probes and couples of primers from one or more of these polymorphic fragments may provide a useful and rapid tool for identifying species of ectomycorrhizal fungi in addition to conventional methods (morphological parameters).

引用

页码：149 / 154

页数：6

共 14 条

[1]

[Anonymous], 1990, PCR protocols: a guide to methods and applications, DOI [10.1016/B978-0-12-372180-8.50038-X, DOI 10.1016/B978-0-12-372180-8.50038-X]

[2] THE USE OF RAPD MARKERS IN THE GENETIC-ANALYSIS OF THE PLANT-PATHOGENIC FUNGUS CLADOSPORIUM-FULVUM [J].