Use of sequence characterised amplified region and RAPD markers in the identification of the white truffle Tuber magnatum Pico

被引:20
作者
Amicucci, A [1 ]
Rossi, I [1 ]
Potenza, L [1 ]
Agostini, D [1 ]
Stocchi, V [1 ]
机构
[1] UNIV URBINO,IST CHIM BIOL,I-61029 URBINO,PS,ITALY
关键词
D O I
10.1023/A:1018493111804
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Isolates of white truffles were identified as Tuber magnatum Pico species using a pair of primers selected from a sequence characterised amplified region (SCAR) and a specific random amplified polymorphic DNA (RAPD) marker. The present study reveals that PCR-fragment-pattern polymorphisms, the construction of probes and couples of primers from one or more of these polymorphic fragments may provide a useful and rapid tool for identifying species of ectomycorrhizal fungi in addition to conventional methods (morphological parameters).
引用
收藏
页码:149 / 154
页数:6
相关论文
共 14 条
[11]   THE UTILITY OF RAPD MARKERS FOR THE DETERMINATION OF GENETIC-VARIATION IN OIL PALM (ELAEIS-GUINEENSIS) [J].
SHAH, FH ;
RASHID, O ;
SIMONS, AJ ;
DUNSDON, A .
THEORETICAL AND APPLIED GENETICS, 1994, 89 (06) :713-718
[12]  
TRAPPE JM, 1979, MYCOTAXON, V9, P297
[13]   FINGERPRINTING GENOMES USING PCR WITH ARBITRARY PRIMERS [J].
WELSH, J ;
MCCLELLAND, M .
NUCLEIC ACIDS RESEARCH, 1990, 18 (24) :7213-7218
[14]   DNA POLYMORPHISMS AMPLIFIED BY ARBITRARY PRIMERS ARE USEFUL AS GENETIC-MARKERS [J].
WILLIAMS, JGK ;
KUBELIK, AR ;
LIVAK, KJ ;
RAFALSKI, JA ;
TINGEY, SV .
NUCLEIC ACIDS RESEARCH, 1990, 18 (22) :6531-6535