Separation of Podophyllum lignans by micellar electrokinetic capillary chromatography (MECC)

被引：11

作者：

Ganzera, M ^{[1
]}

Moraes, RM

Khan, IA

机构：

[1] Univ Mississippi, Sch Pharm, Pharmaceut Sci Res Inst, Natl Ctr Dev Nat Prod, University, MS 38677 USA

[2] Univ Mississippi, Sch Pharm, Dept Pharmacognosy, University, MS 38677 USA

来源：

CHROMATOGRAPHIA | 1999年 / 49卷 / 9-10期

关键词：

micellar electrokinetic chromatography; lignans; Podophyllum peltatum;

D O I：

10.1007/BF02467758

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Separation of the main Podophyllum lignans by MECC has been achieved using a phosphate-borate buffer system with the pH of 7.25, containing 25 mM SDS and 15 % methanol. Optimum temperature and voltage were found to be 35 degrees C and 20 kV, respectively, UV-detection was performed at 220 nm. The method was successfully used to analyse different samples of Podophyllum peltatum collected in the southern USA, and showed great qualitative and quantitative variations among these populations. The quantitative MECC re suits for podophyllotoxin were in good agreement to those obtained by HPLC.

引用

页码：552 / 556

页数：5

共 9 条

[1] Quantitation of aryltetralin lignans in plant parts and among different populations of Podophyllum peltatum by reversed-phase high-performance liquid chromatography [J].