Solubility diagram of the Rhodobacter sphaeroides reaction center as a function of PEG concentration

被引：14

作者：

Gaucher, JF

RiesKautt, M

ReissHusson, F

Ducruix, A

机构：

[1] CNRS,LAB ENZYMOL & BIOCHIM STRUCT,F-91198 GIF SUR YVETTE,FRANCE

[2] CNRS,CTR GENET MOL,F-91198 GIF SUR YVETTE,FRANCE

来源：

FEBS LETTERS | 1997年 / 401卷 / 2-3期

关键词：

beta-octylglucoside; crystallization; solubility; membrane protein; photochemical reaction center; polyethylene glycol;

D O I：

10.1016/S0014-5793(96)01446-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to quantify the effect of polyethylene glycol 4000 (PEG) on the solubility of an integral membrane protein, we have crystallized the photochemical reaction center from Rhodobacter sphaeroides Y by batch method on a large range of PEG, The measurement of the solubility diagram display a semi-logarithmic dependence of solubility versus PEG concentration, Comparison of our results with previously published ones [Odahara, T., Ataka, M. and Katsura, M. (1991) Acta Cryst. D50, 639-642] suggests a notable effect of additional 1,2,3-heptane-triol and/or temperature on photochemical reaction center solubility.

引用

页码：113 / 116

页数：4

共 35 条

[11]

DUCRUIX A, 1988, PHOTOSYNTHETIC BACTE, P21

[12]

DUCRUIX A, 1992, CRYSTALLIZATION NUCL, P90

[13] STRUCTURE OF THE PHOTOSYNTHETIC REACTION-CENTER FROM RHODOBACTER-SPHAEROIDES AT 2.65-ANGSTROM RESOLUTION - COFACTORS AND PROTEIN-COFACTOR INTERACTIONS [J].