The 47 kDa Akt substrate associates with phosphodiesterase 3B and regulates its level in adipocytes

被引：12

作者：

Chavez, JA

Gridley, S

Sano, H

Lane, WS

Lienhard, GE ^{[1
]}

机构：

[1] Dartmouth Med Sch, Dept Biochem, Hanover, NH 03755 USA

[2] Harvard Univ, Dept Mol & Cellular Biol, Harvard Microchem & Proteom Facil, Cambridge, MA 02138 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 342卷 / 04期

关键词：

adipocyte; Akt; insulin; lipolysis; phosphodiesterase; 3B; protein kinase B; gi74193400;

D O I：

10.1016/j.bbrc.2006.02.091

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously described a novel putative 47 kDa substrate for the protein kinase Akt (designated AS47) in 3T3-L1 adipocytes. In the present study, we have found by co-immunoprecipitation that AS47 was associated with cyclic nucleotide phosphodiesterase 3B (PDE3B) in lysates of 3T3-L1 adipocytes. The patterns of expression of AS47 and PDE3B upon 3T3-L1 adipocyte differentiation, among mouse tissues, and in adipocytes with and without the transcription factor C/EBP alpha were virtually coincident. Partial knockdown of AS47 in 3T3-L1 adipocytes with shRNA resulted in a similar reduction in PDE3B protein. These results indicate that AS47 exists in a complex with PDE3B in adipocytes and that the amount of AS47 protein regulates the amount of PDE3B. (c) 2006 Elsevier Inc. All rights reserved.

引用

收藏

页码：1218 / 1222

页数：5

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