Strong antimicrobial activity of Lactobacillus rhamnosus GG against Salmonella typhimurium is due to accumulation of lactic acid

被引：263

作者：

De Keersmaecker, SCJ ^{[1
]}

Verhoeven, TLA ^{[1
]}

Desair, J ^{[1
]}

Marchal, K ^{[1
]}

Vanderleyden, J ^{[1
]}

Nagy, I ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Ctr Microbial & Plant Genet, B-3001 Heverlee, Belgium

来源：

FEMS MICROBIOLOGY LETTERS | 2006年 / 259卷 / 01期

关键词：

Lactobacillus rhamnosus GG; probiotics; Salmonella typhimurium; lactic acid; antimicrobial compound; organic acids; coculture;

D O I：

10.1111/j.1574-6968.2006.00250.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

Spent culture supernatant (SCS) of the probiotic Lactobacillus rhamnosus GG had been reported to exert antibacterial activity against Salmonella typhimurium. However, the chemical identity of the antimicrobial compound(s) responsible remained unknown. A survey of the antimicrobial compounds produced by L. rhamnosus GG was performed. Lactobacillus rhamnosus GG produced a low-molecular weight, heat-stable, non-proteinaceous bactericidal substance, active at acidic pH against a wide range of bacterial species. SCS of L. rhamnosus GG grown in MRS medium contained five compounds that could meet the above description, if present at the appropriate concentration. Based on different experimental approaches, it could be concluded that under the growth conditions tested, the strong antimicrobial activity of L. rhamnosus GG against Salmonella was mediated by lactic acid.

引用

页码：89 / 96

页数：8

共 30 条

[1]

Lactic acid permeabilizes gram-negative bacteria by disrupting the outer membrane [J].

Alakomi, HL ;

Skyttä, E ;

Saarela, M ;

Mattila-Sandholm, T ;

Latva-Kala, K ;

Helander, IM .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (05) :2001-2005

[2]

Instruments of microbial warfare: bacteriocin synthesis, toxicity and immunity [J].

Baba, T ;

Schneewind, O .

TRENDS IN MICROBIOLOGY, 1998, 6 (02) :66-71

[3]

The human Lactobacillus acidophilus strain LA1 secretes a nonbacteriocin antibacterial substance(s) active in vitro and in vivo [J].

BernetCamard, MF ;

Lievin, V ;

Brassart, D ;

Neeser, JR ;

Servin, AL ;

Hudault, S .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (07) :2747-2753

[4]

The beneficial, antimicrobial effect of probiotics [J].

Bongaerts, GPA ;

Severijnen, RSVM .

MEDICAL HYPOTHESES, 2001, 56 (02) :174-177

[5]

Antibacterial effect of the adhering human Lactobacillus acidophilus strain LB [J].

Coconnier, MH ;

Lievin, V ;

BernetCamard, MF ;

Hudault, S ;

Servin, AL .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (05) :1046-1052

[6]

DE MAN J. C., 1960, JOUR APPL BACT, V23, P130, DOI 10.1111/j.1365-2672.1960.tb00188.x

[7]

Inhibition of in vitro growth of enteropathogens by new Lactobacillus isolates of human intestinal origin [J].

Drago, L ;

Gismondo, MR ;

Lombardi, A ;

deHaen, C ;

Gozzini, L .

FEMS MICROBIOLOGY LETTERS, 1997, 153 (02) :455-463

[8]

Expression of the hilA Salmonella typhimurium gene in a poultry Salm. enteritidis isolate in response to lactate and nutrients [J].

Durant, JA ;

Corrier, DE ;

Stanker, LH ;

Ricke, SC .

JOURNAL OF APPLIED MICROBIOLOGY, 2000, 89 (01) :63-69

[9]

THE ANTI-MICROBIAL EFFECT OF DISSOCIATED AND UNDISSOCIATED SORBIC ACID AT DIFFERENT PH LEVELS [J].

EKLUND, T .

JOURNAL OF APPLIED BACTERIOLOGY, 1983, 54 (03) :383-389

[10]

pH-, lactic acid-, and non-lactic acid-dependent activities of probiotic lactobacilli against Salmonella enterica serovar typhimurium [J].

Fayol-Messaoudi, D ;

Berger, UN ;

Coconnier-Polter, MH ;

Liévin-Le Moal, V ;

Servin, AL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (10) :6008-6013

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