Protein density gradients on surfaces

被引：60

作者：

Caelen, I ^{[1
]}

Gao, H ^{[1
]}

Sigrist, H ^{[1
]}

机构：

[1] Ctr Suisse Elect & Microtech SA, CH-2007 Neuchatel, Switzerland

来源：

LANGMUIR | 2002年 / 18卷 / 07期

关键词：

D O I：

10.1021/la0113217

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Gradients of biologically active proteins can be obtained by applying photochemical reactions. A photosensitive polysaccharide-based polymer (OptoDex) is used to covalently immobilize proteins on surfaces. Gradients of proteins are generated by varying the dose of light during the photoimmobilization. Probe proteins conserve their catalytic activity or immunological binding characteristics when linked to surfaces exemplified by silicon nitride or polystyrene. Heterogeneous immunoreactions between photoimmobilized antigens and antibodies showed an optimum binding efficiency at an antigen density of approximatly 1.3 ng/mm(2).

引用

页码：2463 / 2467

页数：5

共 33 条

[31] FACTORS AFFECTING THE SPECIFIC ACTIVITY OF IMMOBILIZED ANTIBODIES AND THEIR BIOLOGICALLY-ACTIVE FRAGMENTS [J].

WIMALASENA, RL ;

WILSON, GS .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1991, 572 (1-2) :85-102

[32] Protein interactions with poly(ethylene glycol) self-assembled monolayers on glass substrates: Diffusion and adsorption [J].

Yang, ZH ;

Galloway, JA ;

Yu, HU .

LANGMUIR, 1999, 15 (24) :8405-8411

[33] Cytochrome c recognition of immobilized, orientational variants of cytochrome b5:: Direct force and equilibrium binding measurements [J].

Yeung, C ;

Purves, T ;

Kloss, AA ;

Kuhl, TL ;

Sligar, S ;

Leckband, D .

LANGMUIR, 1999, 15 (20) :6829-6836

← 1 2 3 4 →