Microfluidic encapsulation of cells in alginate particles via an improved internal gelation approach

被引：61

作者：

Akbari, Samin ^{[1
,2
]}

Pirbodaghi, Tohid ^{[2
,3
]}

机构：

[1] Ecole Polytech Fed Lausanne, Lausanne, Switzerland

[2] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

[3] Univ Bern, Grad Sch Cellular & Biomed Sci, Bern, Switzerland

来源：

MICROFLUIDICS AND NANOFLUIDICS | 2014年 / 16卷 / 04期

基金：

瑞士国家科学基金会;

关键词：

Single cell encapsulation; Alginate; Droplet-based microfluidics; DRUG-DELIVERY; HYDROGEL-BEADS; LIVING CELLS; STEM-CELLS; MICROBEADS; DEVICE; MICROENCAPSULATION; MICROPARTICLES; GENERATION;

D O I：

10.1007/s10404-013-1264-z

中图分类号：

TB3 [工程材料学];

学科分类号：

082905 [生物质能源与材料];

摘要：

An improved internal gelation approach is developed to encapsulate single mammalian cells in monodisperse alginate microbeads as small as 26 mu m in diameter and at rates of up to 1 kHz with high cell viability. The cell damage resulting from contact with calcium carbonate nanoparticles as gelation reagents is eliminated by employing a co-flow microfluidic device, and the cell exposure to low pH is minimized by a chemically balanced off-chip gelation step. These modifications significantly improve the viability of cells encapsulated in gelled alginate particles. Two different mammalian cell types are encapsulated with viability of over 84 %. The cells are functional and continue to grow inside the microparticles.

引用

页码：773 / 777

页数：5

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