Visualization of the degradation of a disulfide polymer, linear poly(ethylenimine sulfide), for gene delivery

被引：175

作者：

Lee, Yan ^{[1
]}

Mo, Heejung ^{[1
]}

Koo, Heebeom ^{[1
]}

Park, Jong-Yeun ^{[1
]}

Cho, Min Yi ^{[1
]}

Jin, Geun-woo ^{[1
]}

Park, Jong-Sang ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Chem & Mol Engn, Seoul 151742, South Korea

来源：

BIOCONJUGATE CHEMISTRY | 2007年 / 18卷 / 01期

关键词：

D O I：

10.1021/bc060113t

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyethylenimine (PEI) shows high transfection efficiency and cytoxicity due to its high amine density. The new disulfide cationic polymer, linear poly(ethylenimine sulfide) (l-PEIS), was synthesized for efficient and safe gene delivery. As the amine density of l-PEIS increased, the transfection efficiency also increased. l-PEIS-6 and l-PEIS-8 show transfection efficiencies that are similar to that of PEI. However, cytotoxicity of l-PEIS was not observed due to the biodegradable disulfide bond. The disulfide bonds are stable in the oxidative extracellular condition and can be degraded rapidly in the reductive intracellular condition. The degradation of l-PEIS in HeLa cells was visualized by fluorescence microscopy using the probe-probe dequenching effect of BODIPY-FL fluorescence dye. l-PEIS was degraded completely within 3 h.

引用

页码：13 / 18

页数：6

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