Uptake of palytoxin-like compounds by shellfish fed Ostreopsis siamensis (Dinophyceae)

Greenshell(TM) mussels (Perna canaliculus Gmelin), scallops (Pecten novaezealandiae Reeve), and Pacific oysters (Crassostrea gigas Thunberg) were fed with a New Zealand strain of mass cultured Ostreopsis siamensis Schmidt (for 27 and 84 h and with 1.5 x 10(6) or 8.6 x 10(6) cells, respectively) under laboratory conditions. The microalgal cells contained 0.3 pg palytoxin equivalents cell(-1) (as determined by the haemolysis neutralisation assay (HNA) of Bignami (1993)) and extracts of these cells were toxic to mice after intraperitoneal injection. No palytoxin-like material was detected either in the hepatopancreas or the muscle and roe of mussels fed O. siamensis. Oysters contained detectable amounts of toxin in hepatopancreas muscle, and roe while higher concentrations were present in the hepatopancreas of scallops. Extracts of control shellfish (tested biotoxin free and not fed O. siamensis) were toxic to mice, and there was no definitive evidence that feeding shellfish with O. siamensis at the levels employed in the present experiment increased the toxicity of shellfish tissue extracts to mice.