Microchip-based CEC of nitroaromatic and nitramine explosives using silica-based sol-gel stationary phases from methyl- and ethyl-trimethoxysilane precursors

被引：30

作者：

Giordano, Braden C.

Terray, Alex

Collins, Greg E.

机构：

[1] USN, Res Lab, Div Chem, Washington, DC 20375 USA

[2] SAIC, Washington, DC USA

来源：

ELECTROPHORESIS | 2006年 / 27卷 / 21期

关键词：

capillary electrokinetic chromatography; microchip electrophoresis; nitroaromatic and nitramine explosives; sol-gel;

D O I：

10.1002/elps.200600254

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Microchip-based CEC of nitroaromatic and nitramine explosives with UV absorbance detection is described. The stationary phase was deposited in the microchip using the sol-gel process. Unique to this work, is the exclusive use of alkylated-trimethoxysilane precursors in the gel solution. Using alkylated precursors allows for the synthesis of a hydrophobic stationary phase in a single step. Three sol-gel formulations of increasing hydrophobicity and suitable for the separation of explosives are established from methyl- and ethyl-trimethoxysilane precursors. Increasing the alkyl-chain length improved the resolution significantly, allowing for the separation of up to seven analytes. Direct injection onto the head of the stationary phase for long injection times, results in sub-mg/L detection limits with little effect on separation efficiency.

引用

页码：4295 / 4302

页数：8

共 54 条

[1]

Allen D, 2004, J CHROMATOGR A, V1038, P299, DOI 10.1016/j.chroma.2004.03.053

[2] Capillary electrochromatography with monolithic silica columns III. Preparation of hydrophilic silica monoliths having surface-bound cyano groups: chromatographic characterization and application to the separation of carbohydrates, nucleosides, nucleic acid bases and other neutral polar species [J].