Aims: To compare procedures for recovering template DNA from ground beef or chicken for polymerase chain reaction (PCR)-based detection of Salmonella. Methods and Results: The primer set of ST11 and ST15 was utilized to amplify a 429-bp product from Salmonella serotype Typhimurium. Boiling and three commercial kits were evaluated for extracting DNA from pure suspensions and artificially contaminated ground beef and chicken. The detection sensitivity of the PCR assay for pure cultures was independent of the template preparation method (P = 0.946). Boiling and GeneReleaser(TM) failed to detect Salm. Typhimurium at 4 x 10(6) cfu g(-1) in ground chicken. PrepMan(TM) Ultra and the high pure PCR template preparation kit facilitated reliable and sensitive detection of Salm. Typhimurium in two types of food. The sensitivities were approx. 4 x 10(3) cfu g(-1). When spiked samples were enriched in peptone water for 6 h, an initial inoculum of 1 cfu g(-1) was detectable. Conclusions: Four template DNA preparation methods differed in performance with respect to the type of samples tested. Significance and Impact of the Study: Template DNA for the PCR detection of pathogenic bacteria, such as Salmonella in meat and poultry, could be effectively obtained using a simple rapid method such as the commercially available PrepMan(TM) Ultra kit.
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Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA
Mead, PS
Slutsker, L
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Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA
Slutsker, L
Dietz, V
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Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA
Dietz, V
McCaig, LF
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Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA
McCaig, LF
Bresee, JS
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Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA
Bresee, JS
Shapiro, C
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Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA
Shapiro, C
Griffin, PM
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Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA
Griffin, PM
Tauxe, RV
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Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA
机构:
Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA
Mead, PS
Slutsker, L
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA
Slutsker, L
Dietz, V
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h-index: 0
机构:
Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA
Dietz, V
McCaig, LF
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h-index: 0
机构:
Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA
McCaig, LF
Bresee, JS
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h-index: 0
机构:
Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA
Bresee, JS
Shapiro, C
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h-index: 0
机构:
Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA
Shapiro, C
Griffin, PM
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h-index: 0
机构:
Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA
Griffin, PM
Tauxe, RV
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USACtr Dis Control & Prevent, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA