Humoral and cellular immune responses in different rat strains on oral exposure to ovalbumin

被引:62
作者
Knippels, LMJ
Penninks, AH
van Meeteren, M
Houben, GF
机构
[1] TNO, Nutr & Food Res Inst, Dept Toxicol, NL-3700 AJ Zeist, Netherlands
[2] Univ Utrecht, Toxicol Res Inst, NL-3508 TD Utrecht, Netherlands
关键词
food allergy; ovalbumin; oral; Brown Norway; Hooded Lister; Wistar; PVG; rat; IgG; IgE; DTH;
D O I
10.1016/S0278-6915(99)00064-2
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
No adequate enteral sensitization models are available to study food allergy and allergenicity of food proteins. Using a previously described oral sensitization protocol to sensitize Brown Norway rats (BN) to food proteins, the influence of genetically-based strain-specific characteristics of the immune system on the outcome of oral sensitization studies was investigated. BN, Hooded Lister (HL), Piebald Virol Glare (PVG) and Wistar rats were daily administered 1 mg of ovalbumin (OVA) by gavage dosing for 42 days without the use of an adjuvants. The highest OVA-specific IgG responses were detected in the BN rats followed by Wistar, HL and PVG rats. OVA-specific IgE responses were only detectable in the BN rats. The cellular immune response was examined by determination of delayed-type hypersensitivity (DTH) reactions in the animals. The response was most pronounced in the HL and Wistar rats. PVG and BN rats showed comparable DTH responses but the responses were significantly weaker than those observed in HL and Wistar rats. It was concluded that the genetic make-up of different rat strains influences the outcome of oral sensitization studies. In addition, using the described oral sensitization protocol, the BN rat seems to be the most suitable strain for inducing oral sensitization. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:881 / 888
页数:8
相关论文
共 34 条
[21]  
MASON D, 1990, IMMUNOLOGY, V70, P1
[22]   MOLECULAR-CLONING OF RAT INTERLEUKIN-4 CDNA AND ANALYSIS OF THE CYTOKINE REPERTOIRE OF SUBSETS OF CD4+ T-CELLS [J].
MCKNIGHT, AJ ;
BARCLAY, AN ;
MASON, DW .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1991, 21 (05) :1187-1194
[23]   Hexachlorobenzene-induced immunomodulation and skin and lung lesions: A comparison between Brown Norway, Lewis, and Wistar rats [J].
Michielsen, CPPC ;
Bloksma, N ;
Ultee, A ;
VanMil, F ;
Vos, JG .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 1997, 144 (01) :12-26
[24]   Altered Th1/Th2 balance associated with nonmajor histocompatibility complex genes in collagen-induced arthritis in resistant and nonresistant rat strains [J].
Mussener, A ;
Lorentzen, JC ;
Kleinau, S ;
Klareskog, L .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1997, 27 (03) :695-699
[25]  
NOBLE A, 1993, IMMUNOLOGY, V79, P562
[26]  
PAHUD JJ, 1985, PRODUCTION REGULATIO, P264
[27]  
PAPP I, 1992, J IMMUNOL, V148, P1308
[28]   ABILITY OF A NEW INFANT FORMULA PREPARED FROM PARTIALLY HYDROLYZED BOVINE WHEY TO INDUCE ANAPHYLACTIC SENSITIZATION - EVALUATION IN A GUINEA-PIG MODEL [J].
PIACENTINI, GL ;
BERTOLINI, A ;
SPEZIA, E ;
PISCIONE, T ;
BONER, AL .
ALLERGY, 1994, 49 (05) :361-364
[29]  
STOKES CR, 1983, CLIN EXP IMMUNOL, V52, P678
[30]   Immune responses to dietary antigens: oral tolerance [J].
Strobel, S ;
Mowat, AM .
IMMUNOLOGY TODAY, 1998, 19 (04) :173-181