An engineered plant that accumulates higher levels of heavy metals than Thlaspi caerulescens, with yields of 100 times more biomass in mine soils

被引:73
作者
Martinez, Mar
Bernal, Pilar
Almela, Concepcion
Velez, Dinoraz
Garcia-Agustin, Pilar
Serrano, Ramon
Navarro-Avino, Juan
机构
[1] CSIC, IBMCP, Dept Stress Biol, Valencia 46022, Spain
[2] CSIC, Ctr Edafol & Appl Biol Segura, Dept Soil & Water Conservat & Organ Waste Managem, Murcia 30100, Spain
[3] Univ Valencia, CSIC, Inst Agr & Food Technol, E-46100 Valencia, Spain
[4] Univ Jaume 1, ESTCE, Area Vegetable Physiol, Dept Expt Sci, Castellon de La Plana 12071, Spain
关键词
boron; Nicotiana glauca; phytochelatin synthase; phytoremediation;
D O I
10.1016/j.chemosphere.2005.10.044
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Nicotiana glauca transformed with TaPCS1 was tested for its application in phytoremediation. When plantlets were grown in mine soils containing Cu, Zn, and Ph (42, 2600, and 1500 mg kg(-1)) the plant showed high levels of accumulation especially of Zn and Pb. Adult plants growing in mine soils containing different heavy metal concentrations showed a greater accumulation as well as an extension to a wider range of elements, including Cd, Ni and B. The overexpressed gene confers up to 9 and 36 times more Cd and Pb accumulation in the shoots under hydroponic conditions, and a 3- and 6-fold increase in mining soils. When the hyperaccumulator Thlaspi caerulescens was compared, the results were higher values of heavy metal and Boron accumulation, with a yield of 100 times more biomass. Thlaspi was unable to survive in mining soils containing either a level higher than 11000 mg kg(-1) of Pb and 4500 mg kg(-1) of Zn, while engineered plants yielded an average of 0.5 kg per plant. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:478 / 485
页数:8
相关论文
共 26 条
[21]   Phytoremediation [J].
Salt, DE ;
Smith, RD ;
Raskin, I .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1998, 49 :643-668
[22]   Engineering tolerance and accumulation of lead and cadmium in transgenic plants [J].
Song, WY ;
Sohn, EJ ;
Martinoia, E ;
Lee, YJ ;
Yang, YY ;
Jasinski, M ;
Forestier, C ;
Hwang, I ;
Lee, Y .
NATURE BIOTECHNOLOGY, 2003, 21 (08) :914-919
[23]  
*USDA, SOIL TAX 4 BAS SYST
[24]  
Vassilev Andon, 2004, ScientificWorldJournal, V4, P9
[25]   The effects of soil amendments on heavy metal bioavailability in two contaminated Mediterranean soils [J].
Walker, DJ ;
Clemente, R ;
Roig, A ;
Bernal, MP .
ENVIRONMENTAL POLLUTION, 2003, 122 (02) :303-312
[26]   The effects of copper and lead on growth and zinc accumulation of Thlaspi caerulescens J. and C. Presl:: Implications for phytoremediation of contaminated soils [J].
Walker, DJ ;
Bernal, MP .
WATER AIR AND SOIL POLLUTION, 2004, 151 (1-4) :361-372