Imaging of vapor plumes produced by matrix assisted laser desorption: A plume sharpening effect

被引：94

作者：

Puretzky, AA ^{[1
]}

Geohegan, DB

Hurst, GB

Buchanan, MV

Luk'yanchuk, BS

机构：

[1] Oak Ridge Natl Lab, Oak Ridge, TN 37831 USA

[2] Russian Acad Sci, Inst Gen Phys, Moscow 117942, Russia

来源：

PHYSICAL REVIEW LETTERS | 1999年 / 83卷 / 02期

关键词：

D O I：

10.1103/PhysRevLett.83.444

中图分类号：

O4 [物理学];

学科分类号：

0702 [物理学];

摘要：

The first gated imaging of both light matrix molecule and heavy biomolecule vapor expansion during matrix assisted laser desorption and ionization is reported, revealing a plume sharpening effect. Laser induced fluorescence imaging of dye-tagged DNase I proteins shows that these heavy molecules (30 000 Da) propagate within a very narrow angular distribution compared to that of the 3-HPA matrix (139 Da). A special solution of the gas dynamic equations is developed to describe the 3D coexpansion of heavy and light plume components.

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收藏

页码：444 / 447

页数：4

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