Peptide-protein microarrays for the simultaneous detection of pathogen infections

被引：77

作者：

Duburcq, X

Olivier, C

Malingue, F

Desmet, R

Bouzidi, A

Zhou, FL

Auriault, C

Gras-Masse, H

Melnyk, O

机构：

[1] Biol Inst Lille, CNRS, UMR 8527, F-59021 Lille, France

[2] Sedac Therapeut Galenis, F-59120 Loos, France

[3] CNRS, Biol Inst Lille, UMR 8525, F-59021 Lille, France

来源：

BIOCONJUGATE CHEMISTRY | 2004年 / 15卷 / 02期

关键词：

D O I：

10.1021/bc034226d

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe novel peptide-protein microarrays, which were fabricated using semicarbazide glass slides that permitted the immobilization of glyoxylyl peptides by site-specific ligation and the immobilization of proteins by physisorption. The arrays permitted the simultaneous serodetection of antibodies directed against hepatitis C virus (HCV core p21 15-45 peptide, NS4 1925-1947 peptide, core, NS3, NS4, and mixture of core, NS3, NS4, and NS5 antigens), hepatitis B virus (HBc, HBe, and HBs), human immunodeficiency virus (Gp41 and Gp120 for HIV-I and Gp36 for HIV-II), Epstein-Barr virus (VCAp18 153-176 peptide), and syphilis (rTpN47 and rTpN17) antigens using an immunofluorescence assay. Peptide-protein microarrays displayed high signal-to-noise ratios, sensitivities, and specificities for the detection of antibodies as revealed by the analysis of a collection of human sera referenced against these five pathogens.

引用

页码：307 / 316

页数：10

共 30 条

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