A sequence-based map of the nine genes of the human interleukin-1 cluster

被引：174

作者：

Nicklin, MJH ^{[1
]}

Barton, JL

Nguyen, M

FitzGerald, MG

Duff, GW

Kornman, K

机构：

[1] Univ Sheffield, Royal Hallamshire Hosp, Div Genom Med, Sheffield S10 2JF, S Yorkshire, England

[2] Genome Therapeut Corp, Waltham, MA 02154 USA

[3] Interleukin Genet Inc, Waltham, MA 02459 USA

来源：

GENOMICS | 2002年 / 79卷 / 05期

关键词：

interleukin-1; gene cluster; family; reference sequence; inflammation; inflammatory cytokine; annotated genetic map;

D O I：

10.1006/geno.2002.6751

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Six novel genes encoding proteins with the interleukin (IL)-1 fold have been identified recently. The classical family members are involved in inflammatory signaling. Previous work has placed the novel genes close to or within the same cluster as IL1A, IL1B, and IL1RN, which occupy an similar to 400-kb interval on chromosome 2. We have combined the incomplete public database sequence with our own sequence to generate a reference sequence and map that encompass all of the novel genes, allowing determination of the gene structures, precise localization of exons, and determination of distances between conventional SNP and microsatellite markers. Gene order from centromere to telomere is IL1A-IL1B-IL1F7-IL1F9-IL1F6-IL1F8-IL1F5-IL1F10-IL1RN, of which only IL1A, IL1B, and IL1F8 are transcribed towards the centromere. The gene order relates to the evolutionary relationship between the genes. Key features of exon boundaries are conserved. There is no evidence for other IL-1 family members within the cluster.

引用

页码：718 / 725

页数：8

共 43 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2]

Barton JL, 2000, EUR J IMMUNOL, V30, P3299, DOI 10.1002/1521-4141(200011)30:11<3299::AID-IMMU3299>3.0.CO

[3]

2-S

[4] A newly defined interleukin-1? [J].

Bazan, JF ;

Timans, JC ;

Kastelein, RA .

NATURE, 1996, 379 (6566) :591-591

[5] Cloning of a novel receptor subunit, AcPL, required for interleukin-18 signaling [J].

Born, TL ;

Thomassen, E ;

Bird, TA ;

Sims, JE .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (45) :29445-29450

[6] Identification and gene organization of three novel members of the IL-1 family on human chromosome 2 [J].

Busfield, SJ ;

Comrack, CA ;

Yu, G ;

Chickering, TW ;

Smutko, JS ;

Zhou, H ;

Leiby, KR ;

Holmgren, LM ;

Gearing, DP ;

Pan, Y .

GENOMICS, 2000, 66 (02) :213-216

[7]

Cox T. S., 1998, Journal of Crop Production, V1, P1, DOI 10.1300/J144v01n01_01

[8] Two novel IL-1 family members, IL-1δ and IL-1ε, function as an antagonist and agonist of NF-κB activation through the orphan IL-1 receptor-related protein 2 [J].

Debets, R ;

Timans, JC ;

Homey, B ;

Zurawski, S ;

Sana, TR ;

Lo, S ;

Wagner, J ;

Edwards, G ;

Clifford, T ;

Menon, S ;

Bazan, JF ;

Kastelein, RA .

JOURNAL OF IMMUNOLOGY, 2001, 167 (03) :1440-1446

[9]

di Giovine FS, 2000, CYTOKINE MOL BIOL, P21

[10] Base-calling of automated sequencer traces using phred.: II.: Error probabilities [J].

Ewing, B ;

Green, P .

GENOME RESEARCH, 1998, 8 (03) :186-194

← 1 2 3 4 5 →