Purification and structural characterization of the thermosome from the hyperthermophilic archaeum Methanopyrus kandleri

被引：41

作者：

Andra, S

Frey, G

Nitsch, M

Baumeister, W

Stetter, KO

机构：

[1] UNIV REGENSBURG,LEHRSTUHL MIKROBIOL,D-93053 REGENSBURG,GERMANY

[2] MAX PLANCK INST BIOCHEM,D-82152 MARTINSRIED,GERMANY

来源：

FEBS LETTERS | 1996年 / 379卷 / 02期

关键词：

Archaea; chaperonin; thermosome; Methanopyrus;

D O I：

10.1016/0014-5793(95)01493-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

From Methanopyras kandleri, the most thermophilic methanogen known so far, we have purified to homogeneity a protein complex of high molecular mass. Image analysis of transmission electron micrographs revealed a barrel-shaped particle composed of two rings with 8-fold symmetry. Only one type of subunit could be detected, The corresponding gene has been cloned and sequenced. The deduced amino acid sequence shows high homology with the members of group II chaperonins. The structure of the projection and the sequence homology suggest that this particle is the first thermosome isolated from a methanogen.

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页码：127 / 131

页数：5

相关论文

共 29 条

[21] TRICINE SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL ELECTROPHORESIS FOR THE SEPARATION OF PROTEINS IN THE RANGE FROM 1-KDA TO 100-KDA [J].

SCHAGGER, H ;

VONJAGOW, G .

ANALYTICAL BIOCHEMISTRY, 1987, 166 (02) :368-379

[22] THE T-COMPLEX POLYPEPTIDE-1 COMPLEX IS A CHAPERONIN FOR TUBULIN AND ACTIN IN-VIVO [J].

STERNLICHT, H ;

FARR, GW ;

STERNLICHT, ML ;

DRISCOLL, JK ;

WILLISON, K ;

YAFFE, MB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (20) :9422-9426

[23] PYRODICTIUM GEN-NOV, A NEW GENUS OF SUBMARINE DISC-SHAPED SULFUR REDUCING ARCHAEBACTERIA GROWING OPTIMALLY AT 105-DEGREES-C [J].

STETTER, KO ;

KONIG, H ;

STACKEBRANDT, E .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 1983, 4 (04) :535-551

[24] ACQUIRED THERMOTOLERANCE AND HEAT-SHOCK IN THE EXTREMELY THERMOPHILIC ARCHAEBACTERIUM SULFOLOBUS SP STRAIN-B12 [J].

TRENT, JD ;

OSIPIUK, J ;

PINKAU, T .

JOURNAL OF BACTERIOLOGY, 1990, 172 (03) :1478-1484

[25] A MOLECULAR CHAPERONE FROM A THERMOPHILIC ARCHAEBACTERIUM IS RELATED TO THE EUKARYOTIC PROTEIN T-COMPLEX POLYPEPTIDE-1 [J].

TRENT, JD ;

NIMMESGERN, E ;

WALL, JS ;

HARTL, FU ;

HORWICH, AL .

NATURE, 1991, 354 (6353) :490-493

[26] PRIMARY STRUCTURE OF THE THERMOSOME FROM THERMOPLASMA-ACIDOPHILUM [J].

WALDMANN, T ;

LUPAS, A ;

KELLERMANN, J ;

PETERS, J ;

BAUMEISTER, W .

BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1995, 376 (02) :119-126

[27] THE THERMOSOME OF THERMOPLASMA-ACIDOPHILUM AND ITS RELATIONSHIP TO THE EUKARYOTIC CHAPERONIN TRIC [J].

WALDMANN, T ;

NIMMESGERN, E ;

NITSCH, M ;

PETERS, J ;

PFEIFER, G ;

MULLER, S ;

KELLERMANN, J ;

ENGEL, A ;

HARTL, FU ;

BAUMEISTER, W .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 227 (03) :848-856

[28] TOWARDS A NATURAL SYSTEM OF ORGANISMS - PROPOSAL FOR THE DOMAINS ARCHAEA, BACTERIA, AND EUCARYA [J].

WOESE, CR ;

KANDLER, O ;

WHEELIS, ML .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (12) :4576-4579

[29] TCP1 COMPLEX IS A MOLECULAR CHAPERONE IN TUBULIN BIOGENESIS [J].

YAFFE, MB ;

FARR, GW ;

MIKLOS, D ;

HORWICH, AL ;

STERNLICHT, ML ;

STERNLICHT, H .

NATURE, 1992, 358 (6383) :245-248