MicroRNA-107 contributes to post-stroke angiogenesis by targeting Dicer-1

被引：115

作者：

Li, Yanan ^{[1
]}

Mao, Ling ^{[1
]}

Gao, Yuan ^{[1
]}

Baral, Suraj ^{[1
]}

Zhou, Yifan ^{[1
]}

Hu, Bo ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Neurol, Union Hosp, Wuhan 430022, Peoples R China

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

中国国家自然科学基金; 新加坡国家研究基金会;

关键词：

SONIC HEDGEHOG; ASTROCYTES; EXPRESSION; OXYGEN;

D O I：

10.1038/srep13316

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Previous studies have suggested that microRNA-107 (miR-107) regulates cell migration in tumor and promotes Hypoxia Inducible Factor 1 alpha (HIF1 alpha) regulated angiogenesis under hypoxia. We found that miR-107 was strongly expressed in ischemic boundary zone (IBZ) after permanent middle cerebral artery occlusion (pMCAO) in rats and inhibition of miR-107 could reduce capillary density in the IBZ after stroke. Such finding led us to hypothesize that miR-107 might regulate post-stroke angiogenesis and therefore serve as a therapeutic target. We also found that antagomir-107, a synthetic miR-107 inhibitor, decreased the number of capillaries in IBZ and increased overall infarct volume after pMCAO in rats. We demonstrated that miR-107 could directly down-regulate Dicer-1, a gene that encodes an enzyme essential for processing microRNA (miRNA) precursors. This resulted in translational desupression of VEGF (vascular endothelial growth factor) mRNA, thereby increasing expression of endothelial cell-derived VEGF (VEGF165/VEGF164), leading to angiogenesis after stroke. This process might be a protective mechanism for ischemia-induced cerebral injury and miR-107 might be used as a novel tool in stroke treatment.

引用

页数：13

共 29 条

[1]

Astrocyte-derived VEGF-A drives blood-brain barrier disruption in CNS inflammatory disease [J].