Markers of glycemic control in the mouse:: comparisons of 6-h- and overnight-fasted blood glucoses to Hb A1c

被引：58

作者：

Han, Byoung Geun ^{[1
]}

Hao, Chuan-Ming ^{[1
]}

Tchekneva, Elena E. ^{[1
]}

Wang, Ying-Ying ^{[1
]}

Lee, Chieh Allen ^{[2
]}

Ebrahim, Benyamin ^{[1
]}

Harris, Raymond C. ^{[1
]}

Kern, Timothy S. ^{[2
]}

Wasserman, David H. ^{[3
,4
]}

Breyer, Matthew D. ^{[1
,3
,4
]}

Qi, Zhonghua ^{[1
,4
]}

机构：

[1] Vanderbilt Univ, Dept Med, Nashville, TN USA

[2] Case Western Reserve Univ, Dept Med, Cleveland, OH 44106 USA

[3] Vanderbilt Univ, Dept Mol Physiol & Biophys, Nashville, TN USA

[4] Vanderbilt Univ, Sch Med, Mouse Metab Phenotyping Ctr, Nashville, TN 37212 USA

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | 2008年 / 295卷 / 04期

关键词：

glycosylated hemoglobin; methodology; mice;

D O I：

10.1152/ajpendo.90283.2008

中图分类号：

R5 [内科学];

学科分类号：

1002 [临床医学]; 100201 [内科学];

摘要：

The present studies examined the relationship between fasting blood glucose and Hb A(1c) in C57BL/6J, DBA/2J, and KK/H1J mice with and without diabetes mellitus. Daily averaged blood glucose levels based on continuous glucose monitoring and effects of 6-h vs. overnight fasting on blood glucose were determined. Daily averaged blood glucose levels were highly correlated with Hb A1c, as determined with a hand-held automated device using an immunodetection method. R-2 values were 0.90, 0.95, and 0.99 in KK/HIJ, C57BL/6J, and DBA/2J, respectively. Six-hour fasting blood glucose correlated more closely with the level of daily averaged blood glucose and with Hb A(1c) than did blood glucose following an overnight fast. To validate the immunoassay-determined Hb A(1c), we also measured total glycosylated hemoglobin using boronate HPLC. Hb A(1c) values correlated well with total glycosylated hemoglobin in all three strains but were relatively lower than total glycosylated hemoglobin in diabetic DBA/2J mice. These results show that 6-h fasting glucose provides a superior index of glycemic control and correlates more closely with Hb A(1c) than overnight-fasted blood glucose in these strains of mice.

引用

页码：E981 / E986

页数：6

共 36 条

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