Genomic characterization of novel marine vesiviruses from Steller sea lions (Eumetopias jubatus) from Alaska

被引:8
作者
McClenahan, Shasta D. [1 ]
Burek, Kathy A. [2 ]
Beckmen, Kimberlee B. [3 ]
Knowles, Nick J. [2 ,4 ]
Neill, John D. [5 ]
Romero, Carlos H. [1 ]
机构
[1] Univ Florida, Coll Vet Med, Dept Infect Dis & Pathol, Gainesville, FL 32610 USA
[2] Alaska Vet Pathol Serv, Eagle River, AK 99577 USA
[3] Alaska Dept Fish & Game, Div Wildlife Conservat, Fairbanks, AK 99701 USA
[4] Inst Anim Hlth, Pirbright Lab, Surrey, England
[5] USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA
基金
美国海洋和大气管理局;
关键词
Caliciviridae; Vesivirus; Steller sea lion; Novel genotypes; Full genomes;
D O I
10.1016/j.virusres.2008.08.002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Marine vesiviruses were isolated in cell culture from oral and rectal swabs and vesicular fluid from Alaskan Steller sea lions (SSL; Eumetopias jubatus). Further characterization by RT-PCR, complete genomic sequencing, and phylogenetic analyses indicated that these viruses are most closely related to the marine vesiviruses, but are distinct viruses and represent two novel genotypes. The complete genome of these two SSL isolates was sequenced after cloning their viral cDNA. The genomes were found to be 8302 and 8305 nucleotides in length, organized in three open reading frames and contained 5' and 3' untranslated regions (UTR) of 19 and 180 nucleotides, respectively. The complete genomes of both SSL viruses were most closely related to each other and shared 83.0% nucleotide identity. Using the very limited number of complete genomic vesivirus sequences available in the NCBI database, these novel SSL vesiviruses seem most closely related to vesicular exanthema of swine virus-A48 and least related to rabbit vesivirus and walrus calicivirus. Specific antiserum against some evolutionary closer marine vesiviruses did not neutralize these isolates supporting the novel nature of these SSL viruses. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:26 / 35
页数:10
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