Assessment of the genotoxicity of contaminated soil with the Allium/Vicia-micronucleus and the Tradescantia-micronucleus assays

被引：91

作者：

Cotelle, S ^{[1
]}

Masfaraud, JF ^{[1
]}

Férard, JF ^{[1
]}

机构：

[1] Univ Metz, Ctr Environm Sci, F-57040 Metz 1, France

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1999年 / 426卷 / 02期

关键词：

genotoxicity; micronucleus assay; contaminated soil; Allium cepa; Vicia faba; Tradescantia;

D O I：

10.1016/S0027-5107(99)00063-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The present study concerns the genotoxicity of contaminated soil near Metz, France. Three plant bioassays, the Vicia faba (broad bean), the Allium cepa (white onion) and the Tradescnntia (spiderwort) micronuclei tests were used to evaluate for genotoxicity. Two soil samples were tested: soil sample A (from an industrial waste site) and soil sample B (from a cokeworks waste site). Maleic hydrazide was used as the positive control. Aqueous extracts of the soil samples were used to treat the roots of Vicia and Allium, and plant cuttings of Tradescantia according to the standard protocol for these plant assays established by the International Program on Chemical Safety and the World Health Organization. The results of these tests showed differential sensitivity in the three different bioassays. Soil sample A was more toxic than soil sample B. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：167 / 171

页数：5

共 15 条

[11] Allium cepa anaphase-telophase root tip chromosome aberration assay on N-methyl-N-nitrosourea, maleic hydrazide, sodium azide, and ethyl methanesulfonate [J].