Identification of native flavin adducts from Fusarium oxysporum using accurate mass matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

被引：15

作者：

Edmondson, RD

Gadda, G

Fitzpatrick, PF

Russell, DH

机构：

[1] TEXAS A&M UNIV,DEPT BIOCHEM & BIOPHYS,COLLEGE STN,TX 77845

[2] TEXAS A&M UNIV,DEPT CHEM,COLLEGE STN,TX 77845

来源：

ANALYTICAL CHEMISTRY | 1997年 / 69卷 / 14期

关键词：

D O I：

10.1021/ac9700989

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Accurate mass matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry is used to determine the identity of naturally occurring substituted flavin adenine dinucleotide (FAD) of nitroalkane oxidase isolated from Fusarium oxysporum. Accurate mass measurements (1-2 ppm) combined with an elemental composition search identifies the unknown FAD substituent as nitrobutane. The use of internal and external calibration in accurate mass MALDI-TOF is discussed.

引用

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页码：2862 / 2865

页数：4

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