Characterization of voltage-gated sodium-channel blockers by electrical stimulation and fluorescence detection of membrane potential

被引：87

作者：

Huang, CJ ^{[1
]}

Harootunian, A ^{[1
]}

Maher, MP ^{[1
]}

Quan, C ^{[1
]}

Raj, CD ^{[1
]}

McCormack, K ^{[1
]}

Numann, R ^{[1
]}

Negulescu, PA ^{[1
]}

González, JE ^{[1
]}

机构：

[1] Vertex Pharmaceut Inc, San Diego, CA 92121 USA

来源：

NATURE BIOTECHNOLOGY | 2006年 / 24卷 / 04期

关键词：

D O I：

10.1038/nbt1194

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Voltage-gated ion channels regulate many physiological functions and are targets for a number of drugs. Patch-clamp electrophysiology is the standard method for measuring channel activity because it fulfils the requirements for voltage control, repetitive stimulation and high temporal resolution, but it is laborious and costly. Here we report an electro-optical technology and automated instrument, called the electrical stimulation voltage ion probe reader (E-VIPR), that measures the activity of voltage-gated ion channels using extracellular electrical field stimulation and voltage-sensitive fluorescent probes. We demonstrate that E-VIPR can sensitively detect drug potency and mechanism of block on the neuronal human type III voltage-gated sodium channel expressed in human embryonic kidney cells. Results are compared with voltage-clamp and show that E-VIPR provides sensitive and information-rich compound blocking activity. Furthermore, we screened similar to 400 drugs and observed sodium channel-blocking activity for similar to 25% of them, including the antidepressants sertraline (Zoloft) and paroxetine (Paxil).

引用

页码：439 / 446

页数：8

共 52 条

[31]

MAHER MP, 2004, Patent No. 6686193

[32]

Maizels M, 2005, AM FAM PHYSICIAN, V71, P483

[33] Systemic lidocaine for neuropathic pain relief [J].