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Use of enzyme penicillin acylase in selective amidation/amide hydrolysis to resolve ethyl 3-amino-4-pentynoate isomers
被引:38
作者:
Topgi, RS
Ng, JS
Landis, B
Wang, P
Behling, JR
机构:
[1] SEARLE, Chem Sci, R&D, Skokie, IL 60077 USA
[2] Monsanto Co, Bioproc Grp, St Louis, MO 63198 USA
关键词:
penicillin acylase;
resolve;
beta-amino acid;
acylation;
deacylation;
D O I:
10.1016/S0968-0896(99)00155-8
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The beta-amino acid, (S)-ethyl-3-amino-4-pentynoate: is a chiral synthon used in the synthesis of xemilofiban hydrochloride, an anti-platelet agent. A biocatalytic approach was developed to resolve (R)- and (S)-enantiomers of ethyl 3-amino-4-pentynoate in enantiomerically pure form employing the enzyme Penicillin acylase. In the acylation, phenylacetic acid was used as an acylating agent. We have shown that both the acylation and deacylation can be employed and that the activity of the enzyme Penicillin acylase can be controlled by maintaining an appropriate pH of the reaction medium. (C) 1999 Elsevier Science Ltd. All rights reserved.
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页码:2221 / 2229
页数:9
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