Graphene/Carbon Nanaotube Aerogel with Ultra-high Adsorption Capacity for the Isolation of Hemoglobin

3-Dimensional graphene oxide/carbon nanotubes (GO/CNTs) aerogel was prepared via freeze drying technique, and the as-prepared GO/CNTs aerogel exhibits excellent adsorption performance towards protein species under hydrophobic interaction. At pH = 8. 0, high adsorption efficiency of hemoglobin (Hb) was achieved, while virtually no adsorption of serum album was observed. This offers the possibility to realize the selective isolation of Fib from complex matrices. An adsorption capacity up to 3793. 3 mg/g was achieved for the GO/CNTs aerogel, suggesting the great potential of this aerogel in the removal of abundant Hb. The adsorbed Hb could be readily stripped using Britton-Robinson buffer of pH= 11. 5, giving rise to a recovery of 67%. Circular dichroism spectra indicated that the adsorption/desorption on the surface of GO/CNTs aerogel poses virtually no effect on the configuration of Hb. The GO/CNTs aerogel is practically applied to the selective isolation of Hb from human blood and SDS-PAGE assays demonstrate high purity of hemoglobin in the eluate.