Isolation of hemoglobin from human blood using solid phase extraction with lanthanum(III) modified zeolite

被引：19

作者：

Chen, Xuwei ^{[1
]}

Chen, Shuai ^{[1
]}

Liu, Jiawei ^{[1
]}

Wang, Jianhua ^{[1
]}

机构：

[1] Northeastern Univ, Res Ctr Analyt Sci, Shenyang 110004, Peoples R China

来源：

MICROCHIMICA ACTA | 2009年 / 165卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Hemoglobin; Isolation; Lanthanum modified clinoptilolite; Solid phase extraction; ION AFFINITY-CHROMATOGRAPHY; PROTEIN-PURIFICATION; BINDING; PRECIPITATION; SEPARATION;

D O I：

10.1007/s00604-008-0123-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

A direct solid-phase extraction procedure for the selective isolation of hemoglobin from complex matrices using lanthanum(III) modified zeolite (clinoptilolite) as sorbent was developed. The modified clinoptilolite provides a sorption capacity of 31.8 mg g(-1) for hemoglobin (Hb). Experimental parameters governing the sorption efficiency, i.e., the pH of sample solution, the ionic strength and sorption time, were investigated. A sorption efficiency of 98% was achieved for 500 ng mu L-1 Hb in 500 mu L of sample solution. The absorbed Hb could be readily stripped by using 500 mu L of a 0.01 mol L-1 orthophosphate solution, giving rise to a recovery of about 80%. The applicability of this method was demonstrated by processing human whole blood for the isolation of Hb, and satisfactory results were obtained by assay with SDS-PAGE.

引用

页码：217 / 222

页数：6

共 21 条

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