Adipose-Derived Stromal Cell Therapy Improves Cardiac Function After Coronary Occlusion in Rats

被引:18
作者
Bagno, Luiza L. S.
Werneck-de-Castro, Joao Pedro S. [2 ]
Oliveira, Patricia F. [3 ]
Cunha-Abreu, Marcia S.
Rocha, Nazareth N. [5 ]
Kasai-Brunswick, Tais H.
Lago, Vivian M.
Goldenberg, Regina C. S.
Campos-de-Carvalho, Antonio C. [1 ,4 ,6 ]
机构
[1] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, CCS, Ilha Fundao, BR-21941902 Rio De Janeiro, RJ, Brazil
[2] Univ Fed Rio de Janeiro, Dept Biociencias Atividade Fis, BR-21941902 Rio De Janeiro, RJ, Brazil
[3] Univ Fed Uberlandia, Inst Ciencias Biomed, Area Ciencias Fisiol, Uberlandia, MG, Brazil
[4] Inst Nacl Cardiol, Rio De Janeiro, RJ, Brazil
[5] Univ Fed Fluminense, Inst Biomed, Rio De Janeiro, RJ, Brazil
[6] Albert Einstein Coll Med, Bronx, NY 10467 USA
关键词
Adipose-derived stromal cell; Myocardial infarction; Cell therapy and heart healing; MESENCHYMAL STEM-CELLS; ACUTE MYOCARDIAL-INFARCTION; BONE-MARROW; IN-VITRO; CARDIOVASCULAR-DISEASES; SYSTOLIC FUNCTION; TEMPORAL-CHANGES; TISSUE; HEART; TRANSPLANTATION;
D O I
10.3727/096368912X636858
中图分类号
Q813 [细胞工程];
学科分类号
100113 [医学细胞生物学];
摘要
Recent studies have identified adipose tissue as a new source of mesenchymal stem cells for therapy. The purpose of this study was to investigate the therapy with adipose-derived stromal cells (ASCs) in a rat model of healed myocardial infarction (MI). ASCs from inguinal subcutaneous adipose tissue of male Wistar rats were isolated by enzymatic digestion and filtration. Cells were then cultured until passage 3. Four weeks after ligation of the left coronary artery of female rats, a suspension of either 100 mu l with phosphate-buffered saline (PBS)+Matrigel+2x10(6) ASCs labeled with Hoechst (n=11) or 100 mu l of PBS+Matrigel (n=10) was injected along the borders of the ventricular wall scar tissue. A sham-operated group (n=5) was submitted to the same surgical procedure except permanent ligation of left coronary artery. Cardiac performance was assessed by electro- and echocardiogram. Echo was performed prior to injections (baseline. BL) and 6 weeks after injections (follow-up, FU), and values after treatment were normalized by values obtained before treatment. Hemodynamic measurements were performed 6 weeks after injections. All infarcted animals exhibited cardiac function impairment. Ejection fraction (EF), shortening fractional area (SFA), and left ventricular akinesia (LVA) were similar between infarcted groups before treatment. Six weeks after therapy. ASC group showed significant improvement in all three ECHO indices in comparison to vehicle group. In anesthetized animals dp/dt(+) was also significantly higher in ASCs when compared to vehicle. In agreement with functional improvement, scar area was diminished in the ASC group. We conclude that ASCs improve cardiac function in infarcted rats when administered directly to the myocardium.
引用
收藏
页码:1985 / 1996
页数:12
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