Production, analysis, and application of spatially resolved shells in solid-phase polymer spheres

被引：32

作者：

Farrer, RA ^{[1
]}

Copeland, GT ^{[1
]}

Previte, MJR ^{[1
]}

Okamoto, MM ^{[1
]}

Miller, SJ ^{[1
]}

Fourkas, JT ^{[1
]}

机构：

[1] Boston Coll, Eugene F Merkert Chem Ctr, Chestnut Hill, MA 02467 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 09期

关键词：

D O I：

10.1021/ja011675k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Two-photon fluorescence microscopy has been used to interrogate the interior functionality of polymer resin beads. By employing this technique, the spatial distribution of the initial functionality contained within the polymer matrix has been determined. Spatially resolved, concentric shells were then produced synthetically in these polymer spheres via a series of protection/deprotection reactions in which two-photon fluorescence microscopy was employed to monitor each successive step. To demonstrate the potential utility of these techniques in combinatorial screening, a set of beads was prepared containing a unique tripeptide sequence in each of the three concentric shells within each individual bead. The set was then screened for the binding affinity of each tripeptide toward a fluorescent ligand.

引用

页码：1994 / 2003

页数：10

共 26 条

[1]

*CALB NOV CORP, 2000, NOV CAT CALB NOV COR

[2] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY [J].

DENK, W ;

STRICKLER, JH ;

WEBB, WW .

SCIENCE, 1990, 248 (4951) :73-76

[3] Design, synthesis, and evaluation of small-molecule libraries [J].

Ellman, JA .

ACCOUNTS OF CHEMICAL RESEARCH, 1996, 29 (03) :132-143

[4] Single-molecule detection with a two-photon fluorescence microscope with fast-scanning capabilities and polarization sensitivity [J].

Farrer, RA ;

Previte, MJR ;

Olson, CE ;

Peyser, LA ;

Fourkas, JT ;

So, PTC .

OPTICS LETTERS, 1999, 24 (24) :1832-1834

[5] Organic chemistry on solid supports [J].

Fruchtel, JS ;

Jung, G .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1996, 35 (01) :17-42

[6] GENERAL-METHOD FOR RAPID SYNTHESIS OF MULTICOMPONENT PEPTIDE MIXTURES [J].

FURKA, A ;

SEBESTYEN, F ;

ASGEDOM, M ;

DIBO, G .

INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, 1991, 37 (06) :487-493

[7] Diffusion of reagents in macrobeads [J].

Groth, T ;

Grotli, M ;

Meldal, M .

JOURNAL OF COMBINATORIAL CHEMISTRY, 2001, 3 (05) :461-468

[8] Solid-phase organic reactions: A review of the recent literature [J].

Hermkens, PHH ;

Ottenheijm, HCJ ;

Rees, D .

TETRAHEDRON, 1996, 52 (13) :4527-4554

[9] Comparison of conventional, confocal, and two-photon microscopy for detection of microcrystals within luminescence-based oxygen sensor films [J].

Kneas, KA ;

Demas, JN ;

DeGraff, BA ;

Periasamy, A .

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES, 2001, 4262 :89-97

[10] The ''one-bead-one-compound'' combinatorial library method [J].

Lam, KS ;

Lebl, M ;

Krchnak, V .

CHEMICAL REVIEWS, 1997, 97 (02) :411-448

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