Single-molecule detection with a two-photon fluorescence microscope with fast-scanning capabilities and polarization sensitivity

被引:21
作者
Farrer, RA [1 ]
Previte, MJR
Olson, CE
Peyser, LA
Fourkas, JT
So, PTC
机构
[1] Boston Coll, Eugene F Merkert Chem Ctr, Chestnut Hill, MA 02467 USA
[2] MIT, Dept Mech Engn, Cambridge, MA 02139 USA
关键词
D O I
10.1364/OL.24.001832
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We describe a laser-scanning two-photon fluorescence microscope that is capable of observing single molecules with excellent temporal resolution and three-dimensional spatial resolution. To demonstrate the capabilities of the instrument we present. single-molecule fluorescence data obtained in several different scanning modes. In addition, a polarization-sensitive detection scheme can provide detailed three-dimensional information about the orientations of molecules in any of these scanning modes. (C) 1999 Optical Society of America OCIS codes: 180.2520, 190.4180.
引用
收藏
页码:1832 / 1834
页数:3
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