Imaging individual molecules by two-photon excitation

被引：26

作者：

Sonnleitner, M ^{[1
]}

Schütz, GJ ^{[1
]}

Schmidt, T ^{[1
]}

机构：

[1] Univ Linz, Inst Biophys, A-4040 Linz, Austria

来源：

CHEMICAL PHYSICS LETTERS | 1999年 / 300卷 / 1-2期

关键词：

D O I：

10.1016/S0009-2614(98)01330-X

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Wide-field epi-illumination of supported lipid membranes by a femtosecond near-infrared laser allowed us to identify and follow individual fluorescence labeled lipids in their motion within the membrane. The fluorophores were excited by two-photon absorption of the near-infrared beam as seen from a quadratic dependence of the fluorescence signal on the illumination intensity. The largely decreased background signal on two-photon excitation compared to that using one-photon excitation suggests that single-molecule imaging on native cell membranes will come into reach. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：221 / 226

页数：6

共 27 条

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Basche T., 1996, SINGLE MOL OPTICAL D

[2] SINGLE MOLECULES OBSERVED BY NEAR-FIELD SCANNING OPTICAL MICROSCOPY [J].