Early Stage Foreign Body Reaction Against Biodegradable Polymer Scaffolds Affects Tissue Regeneration During the Autologous Transplantation of Tissue-Engineered Cartilage in the Canine Model

被引：70

作者：

Asawa, Yukiyo ^{[1
,2
]}

Sakamoto, Tomoaki ^{[1
,2
]}

Komura, Makoto ^{[3
]}

Watanabe, Makoto ^{[1
,2
]}

Nishizawa, Satoru ^{[1
]}

Takazawa, Yutaka ^{[2
,4
]}

Takato, Tsuyoshi ^{[5
,6
]}

Hoshi, Kazuto ^{[1
,2
]}

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Cartilage, Tokyo 1138655, Japan

[2] Univ Tokyo, Grad Sch Med, Dept Bone Regenerat Fujisoft, Tokyo 1138655, Japan

[3] Univ Tokyo, Grad Sch Med, Dept Pediat Surg, Tokyo 1138655, Japan

[4] Tokyo Univ Hosp, Dept Pathol, Tokyo 113, Japan

[5] Univ Tokyo, Grad Sch Med, Dept Sensory, Tokyo 1138655, Japan

[6] Univ Tokyo, Grad Sch Med, Dept Motor Syst Med, Tokyo 1138655, Japan

来源：

CELL TRANSPLANTATION | 2012年 / 21卷 / 07期

关键词：

Autologous chondrocyte transplantation; Canine; Tissue engineering; Biodegradable polymer; HUMAN AURICULAR CHONDROCYTES; IN-VITRO; ARTICULAR CHONDROCYTES; HYBRID SCAFFOLD; DEGRADATION; REPAIR; VIVO; OSTEOARTHRITIS; IMPLANTATION; COMBINATION;

D O I：

10.3727/096368912X640574

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

To overcome the weak points of the present cartilage regenerative medicine, we applied a porous scaffold for the production of tissue-engineered cartilage with a greater firmness and a 3D structure. We combined the porous scaffolds with atelocollagen to retain the cells within the porous body. We conducted canine autologous chondrocyte transplants using biodegradable poly-L-lactic acid (PLLA) or poly-DL-lactic-co-glycolic acid (PLGA) polymer scaffolds, and morphologically and biochemically evaluated the time course changes of the transplants. The histological findings showed that the tissue-engineered constructs using PLLA contained abundant cartilage 1, 2, and 6 months after transplantation. However, the PLGA constructs did not possess cartilage and could not maintain their shapes. Biochemical measurement of the proteoglycan and type II collagen also supported the superiority of PLLA. The biodegradation of PLGA progressed much faster than that of PLLA, and the PLGA had almost disappeared by 2 months. The degraded products of PLGA may evoke a more severe tissue reaction at this early stage of transplantation than PLLA. The PLLA scaffolds were suitable for cartilage tissue engineering under immunocompetent conditions, because of the retarded degradation properties and the decrease in the severe tissue reactions during the early stage of transplantation.

引用

页码：1431 / 1442

页数：12

共 34 条

[1]

Avocado soybean unsaponifiables (ASU) suppress TNF-α, IL-1β, COX-2, iNOS gene expression, and prostaglandin E2 and nitric oxide production in articular chondrocytes and monocyte/macrophages [J].