The Arabidopsis Mediator Subunit MED25 Differentially Regulates Jasmonate and Abscisic Acid Signaling through Interacting with the MYC2 and ABI5 Transcription Factors

被引：318

作者：

Chen, Rong ^{[1
]}

Jiang, Hongling ^{[1
]}

Li, Lin ^{[1
]}

Zhai, Qingzhe ^{[1
]}

Qi, Linlin ^{[1
]}

Zhou, Wenkun ^{[1
]}

Liu, Xiaoqiang ^{[1
]}

Li, Hongmei ^{[1
]}

Zheng, Wenguang ^{[1
]}

Sun, Jiaqiang ^{[1
]}

Li, Chuanyou ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res Beijing, State Key Lab Plant Genom, Beijing 100101, Peoples R China

来源：

PLANT CELL | 2012年 / 24卷 / 07期

基金：

中国国家自然科学基金;

关键词：

METHYL JASMONATE; II TRANSCRIPTION; GENE-EXPRESSION; PSEUDOMONAS-SYRINGAE; DEFENSE RESPONSES; PLANT DEVELOPMENT; OXYLIPIN SIGNAL; THALIANA; COMPLEX; GROWTH;

D O I：

10.1105/tpc.112.098277

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcriptional regulation plays a central role in plant hormone signaling. At the core of transcriptional regulation is the Mediator, an evolutionarily conserved, multisubunit complex that serves as a bridge between gene-specific transcription factors and the RNA polymerase machinery to regulate transcription. Here, we report the action mechanisms of the MEDIATOR25 (MED25) subunit of the Arabidopsis thaliana Mediator in regulating jasmonate- and abscisic acid (ABA)-triggered gene transcription. We show that during jasmonate signaling, MED25 physically associates with the basic helix-loophelix transcription factor MYC2 in promoter regions of MYC2 target genes and exerts a positive effect on MYC2-regulated gene transcription. We also show that MED25 physically associates with the basic Leu zipper transcription factor ABA-INSENSITIVE5 (ABI5) in promoter regions of ABI5 target genes and shows a negative effect on ABI5-regulated gene transcription. Our results reveal that underlying the distinct effects of MED25 on jasmonate and ABA signaling, the interaction mechanisms of MED25 with MYC2 and ABI5 are different. These results highlight that the MED25 subunit of the Arabidopsis Mediator regulates a wide range of signaling pathways through selectively interacting with specific transcription factors.

引用

页码：2898 / 2916

页数：19

共 101 条

[1] Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling [J].