Mining an Ostrinia nubilalis midgut expressed sequence tag (EST) library for candidate genes and single nucleotide polymorphisms (SNPs)

被引：37

作者：

Coates, B. S. ^{[1
]}

Sumerford, D. V. ^{[1
,2
]}

Hellmich, R. L. ^{[1
,2
]}

Lewis, L. C. ^{[1
,2
]}

机构：

[1] Iowa State Univ, USDA, ARS, CICGRU, Ames, IA 50011 USA

[2] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA

来源：

INSECT MOLECULAR BIOLOGY | 2008年 / 17卷 / 06期

关键词：

expressed sequence tag; single nucleotide polymorphism;

D O I：

10.1111/j.1365-2583.2008.00833.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genes expressed in lepidopteran midgut tissues are involved in digestion and Bacillus thuringiensis (Bt) toxin resistance traits. Five hundred and thirty five unique transcripts were annotated from 1745 high quality O. nubilalis larval midgut expressed sequence tags (ESTs). Full-length cDNA sequence of 12 putative serine proteinase genes and 3 partial O. nubilalis aminopeptidase N protein genes, apn1, apn3, and apn4, were obtained, and genes may have roles in plant feeding and Bt toxin resistance traits of Ostrinia larvae. The EST library was not normalized and insert frequencies reflect transcript levels under the initial treatment conditions and redundancy of inserts from highly expressed transcripts allowed prediction of putative single nucleotide polymorphisms (SNPs). Ten di-, tri- or tetranucleotide repeat unit microsatellite loci were identified, and minisatellite repeats were observed within the C-termini of two encoded serine proteinases. Molecular markers showed polymorphism at 28 SNP loci and one microsatellite locus, and Mendelian inheritance indicated that markers were applicable to genome mapping applications. This O. nubilalis larval midgut EST collection is a resource for gene discovery, expression information, and allelic variation for use in genetic marker development.

引用

页码：607 / 620

页数：14

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