Voltammetric microanalysis of DNA adducts with osmium tetroxide,2,2′-bipyridine using a pyrolytic graphite electrode

被引：82

作者：

Fojta, M ^{[1
]}

Havran, L ^{[1
]}

Kizek, R ^{[1
]}

Billová, S ^{[1
]}

机构：

[1] Acad Sci Czech Republ, Inst Biophys, CZ-61265 Brno, Czech Republic

来源：

TALANTA | 2002年 / 56卷 / 05期

关键词：

chemically modified DNA; osmium tetroxide; voltammetry; pyrolytic graphite electrode; DNA labeling;

D O I：

10.1016/S0039-9140(01)00660-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

DNA and synthetic polynucleotides modified with a complex of osmium tetroxide with 2,2'-bipyridine (Os,bipy) produce specific voltammetric signals at pyrolytic graphite electrodes. Based on a sufficient potential separation between the peaks of Os,bipy-modified DNA (DNA-Os,bipy) and of free Os,bipy, and using an adsorptive transfer stripping voltammetric procedure involving extraction of free Os,bipy from the electrode by chloroform, DNA-Os,bipy can be determined in an excess of the free reagent. Under certain conditions, 140 pg of DNA-Os,bipy can be detected after a 5 min accumulation period. This analysis displays a more favorable sensitivity and a better selectivity for DNA structure than oxidation of DNA guanine moieties, and offers detection of osmium DNA markers at carbon electrodes. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：867 / 874

页数：8

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