CopY-like copper inducible repressors are putative 'winged helix' proteins

被引:43
作者
Portmann, R
Poulsen, KR
Wimmer, R
Solioz, M
机构
[1] Univ Bern, Dept Clin Pharmacol, CH-3010 Bern, Switzerland
[2] Aalborg Univ, Dept Phys & Nanotechnol, DK-9000 Aalborg, Denmark
[3] Aalborg Univ, Dept Life Sci, DK-9000 Aalborg, Denmark
关键词
copper homeostasis; winged helix proteins; repressor; Lactobacilli; regulation; surface plasmon resonance;
D O I
10.1007/s10534-005-5381-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
CopY of Enterococcus hirae is a well characterized copper-responsive repressor involved in copper homeostasis. In the absence of copper, it binds to the promoter. In high copper, the CopZ copper chaperone donates copper to CopY, thereby releasing it from the promoter and allowing transcription of the downstream copper homeostatic genes of the cop operon. We here show that the CopY-like repressors from E. hirae, Lactococcus lactis, and Streptococcus mutans have similar affinities not only for their native promoters, but also for heterologous cop promoters. CopZ of L. lactis accelerated the release of CopY from the promoter, suggesting that CopZ of L. lactis acts as copper chaperone, similar to CopZ in E. hirae. The consensus binding motif of the CopY-like repressors was shown to be TACAxxTGTA. The same binding motif is present in promoters controlled by BlaI of Bacillus licheniformis, MecI of Staphylococcus aureus and related repressors. BlaI and MecI have known structures and belong to the family of 'winged helix' proteins. In the N- terminal domain, they share significant sequence similarity with CopY of E. hirae. Moreover, they bind to the same TACAxxTGTA motif. NMR analysis of the N- terminal DNA binding domain of CopY of L. lactis showed that it contained the same alpha-helical content like the same regions of BlaI and MecI. These findings suggest that the DNA binding domains of CopY-like repressors are also of the 'winged helix' type.
引用
收藏
页码:61 / 70
页数:10
相关论文
共 24 条
  • [1] THE PROGRAM XEASY FOR COMPUTER-SUPPORTED NMR SPECTRAL-ANALYSIS OF BIOLOGICAL MACROMOLECULES
    BARTELS, C
    XIA, TH
    BILLETER, M
    GUNTERT, P
    WUTHRICH, K
    [J]. JOURNAL OF BIOMOLECULAR NMR, 1995, 6 (01) : 1 - 10
  • [2] Molecular basis of metal-ion selectivity and zeptomolar sensitivity by CueR
    Changela, A
    Chen, K
    Xue, Y
    Holschen, J
    Outten, CE
    O'Halloran, TV
    Mondragón, A
    [J]. SCIENCE, 2003, 301 (5638) : 1383 - 1387
  • [3] The Enterococcus hirae copper chaperone CopZ delivers copper(I) to the CopY repressor
    Cobine, P
    Wickramasinghe, WA
    Harrison, MD
    Weber, T
    Solioz, M
    Dameron, CT
    [J]. FEBS LETTERS, 1999, 445 (01): : 27 - 30
  • [4] Copper transfer from the Cu(I) chaperone, CopZ, to the repressor, Zn(II)CopY: Metal coordination environments and protein interactions
    Cobine, PA
    George, GN
    Jones, CE
    Wickramasinghe, WA
    Solioz, M
    Dameron, CT
    [J]. BIOCHEMISTRY, 2002, 41 (18) : 5822 - 5829
  • [5] Winged helix proteins
    Gajiwala, KS
    Burley, SK
    [J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 2000, 10 (01) : 110 - 116
  • [6] On the transcriptional regulation of methicillin resistance -: MecI repressor in complex with its operator
    García-Castellanos, R
    Mallorquí-Fernández, G
    Marrero, A
    Potempa, J
    Coll, M
    Gomis-Rüth, FX
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (17) : 17888 - 17896
  • [7] Three-dimensional structure of MecI -: Molecular basis for transcriptional regulation of staphylococcal methicillin resistance
    García-Castellanos, R
    Marrero, A
    Mallorquí-Fernández, G
    Potempa, J
    Coll, M
    Gomis-Rüth, FX
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (41) : 39897 - 39905
  • [8] Studies of the repressor (Blal) of beta-lactamase synthesis in Staphylococcus aureus
    Gregory, PD
    Lewis, RA
    Curnock, SP
    Dyke, KGH
    [J]. MOLECULAR MICROBIOLOGY, 1997, 24 (05) : 1025 - 1037
  • [9] CU(CH3CN)2, EIN MITTEL ZUM STUDIUM HOMOGENER REAKTIONEN DES EINWERTIGEN KUPFERS IN WASSRIGER LOSUNG
    HEMMERICH, P
    SIGWART, C
    [J]. EXPERIENTIA, 1963, 19 (09): : 488 - &
  • [10] MOLMOL: A program for display and analysis of macromolecular structures
    Koradi, R
    Billeter, M
    Wuthrich, K
    [J]. JOURNAL OF MOLECULAR GRAPHICS, 1996, 14 (01): : 51 - &