Ammonia-oxidizing archaea: important players in paddy rhizosphere soil?

被引：339

作者：

Chen, Xue-Ping ^{[1
]}

Zhu, Yong-Guan ^{[1
]}

Xia, Yue ^{[1
]}

Shen, Ju-Pei ^{[1
]}

He, Ji-Zheng ^{[1
]}

机构：

[1] Chinese Acad Sci, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China

来源：

ENVIRONMENTAL MICROBIOLOGY | 2008年 / 10卷 / 08期

关键词：

D O I：

10.1111/j.1462-2920.2008.01613.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The diversity (richness and community composition) of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in paddy soil with different nitrogen (N) fertilizer amendments for 5 weeks were investigated using quantitative real-time polymerase chain reaction, denaturing gradient gel electrophoresis (DGGE) jand clone library analysis based on the ammonia monooxygenase alpha-subunit (amoA) gene. Ammonia-oxidizing archaea predominated among ammonia-oxidizing prokaryotes in the paddy soil, and the AOA:AOB DNA-targeted amoA gene ratios ranged from 1.2 to 69.3. Ammonia-oxidizing archaea were more abundant in the rhizosphere than in bulk soil. Rice cultivation led to greater abundance of AOA than AOB amoA gene copies and to differences in AOA and AOB community composition. These results show that AOA is dominant in the rhizosphere paddy soil in this study, and we assume that AOA were influenced more by exudation from rice root (e.g. oxygen, carbon dioxide) than AOB.

引用

页码：1978 / 1987

页数：10

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