From ANA to ENA: How to proceed?

被引:84
作者
Damoiseaux, JGMC [1 ]
Tervaert, JWC [1 ]
机构
[1] Univ Hosp Maastricht, Dept Clin & Expt Immunol, NL-6229 HX Maastricht, Netherlands
关键词
anti-nuclear antibodies; extractable nuclear antigens; methods; clinical evaluation;
D O I
10.1016/j.autrev.2005.05.007
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Anti-nuclear antibodies (ANA), as detected by indirect immuno-fluorescence, are hallmarks of autoimmune connective tissue diseases. Identification of the specificity for extractable nuclear antigens (ENA) is warranted because this may further differentiate between the distinct types of autoimmune connective tissue diseases. In recent years several different ENA, as recognized by ANA, have been identified and the knowledge of the molecular structure has been expanded. Together with technical developments this has enabled the introduction of several new anti-ENA antibody detection systems. In this review we will discuss the main logistic aspects of anti-ENA antibody testing that have to be solved in order to come to a consensus in order to deal with new developments in this field. We conclude that: 1. a positive ANA test should, depending on the titre and pattern, be followed by an anti-ENA antibody assay, 2: to fully appreciate the value of the new anti-ENA antibody detection systems a large, multicenter clinical evaluation is required, and 3: proper interpretation of reported test results requires that the clinician is aware of the way anti-ENA antibodies are detected and reported. (C) 2005 Elsevier B.V All rights reserved.
引用
收藏
页码:10 / 17
页数:8
相关论文
共 23 条
[1]   Development of autoantibodies before the clinical onset of systemic lupus erythematosus [J].
Arbuckle, MR ;
McClain, MT ;
Rubertone, MV ;
Scofield, RH ;
Dennis, GJ ;
James, JA ;
Harley, JB .
NEW ENGLAND JOURNAL OF MEDICINE, 2003, 349 (16) :1526-1533
[2]   THE AMERICAN-RHEUMATISM-ASSOCIATION 1987 REVISED CRITERIA FOR THE CLASSIFICATION OF RHEUMATOID-ARTHRITIS [J].
ARNETT, FC ;
EDWORTHY, SM ;
BLOCH, DA ;
MCSHANE, DJ ;
FRIES, JF ;
COOPER, NS ;
HEALEY, LA ;
KAPLAN, SR ;
LIANG, MH ;
LUTHRA, HS ;
MEDSGER, TA ;
MITCHELL, DM ;
NEUSTADT, DH ;
PINALS, RS ;
SCHALLER, JG ;
SHARP, JT ;
WILDER, RL ;
HUNDER, GG .
ARTHRITIS AND RHEUMATISM, 1988, 31 (03) :315-324
[3]   Variability between methods to determine ANA, anti-dsDNA and anti-ENA autoantibodies: a collaborative study with the biomedical industry [J].
Bizzaro, N ;
Tozzoli, R ;
Tonutti, E ;
Piazza, A ;
Manoni, F ;
Ghirardello, A ;
Bassetti, D ;
Villalta, D ;
Pradella, M ;
Rizzotti, P .
JOURNAL OF IMMUNOLOGICAL METHODS, 1998, 219 (1-2) :99-107
[4]   Autoantibody profiles in the sera of European patients with myositis [J].
Brouwer, R ;
Hengstman, GJD ;
Egberts, WV ;
Ehrfeld, H ;
Bozic, B ;
Ghirardello, A ;
Grondal, G ;
Hietarinta, M ;
Isenberg, D ;
Kalden, JR ;
Lundberg, I ;
Moutsopoulos, H ;
Roux-Lombard, P ;
Vencovsky, J ;
Wikman, A ;
Seelig, HP ;
van Engelen, BGM ;
van Venrooij, WJ .
ANNALS OF THE RHEUMATIC DISEASES, 2001, 60 (02) :116-123
[5]  
CLARK G, 1969, J IMMUNOL, V102, P117
[6]  
DAMOISEAUX J, IN PRESS ANN N Y ACA
[7]   THE USE OF IMMUNOBLOTTING TO DETECT ANTIBODIES TO NUCLEAR AND CYTOPLASMIC ANTIGENS - CLINICAL AND SEROLOGICAL ASSOCIATIONS IN RHEUMATIC DISEASES [J].
DEROOIJ, DJ ;
VANDEPUTTE, LB ;
HABETS, WJ ;
VERBEEK, AL ;
VANVENROOIJ, WJ .
SCANDINAVIAN JOURNAL OF RHEUMATOLOGY, 1988, 17 (05) :353-364
[8]   DETECTION OF AUTOANTIBODIES TO SS-A/RO BY INDIRECT IMMUNOFLUORESCENCE USING A TRANSFECTED AND OVEREXPRESSED HUMAN 60 KD RO AUTOANTIGEN IN HEP-2 CELLS [J].
FRITZLER, MJ ;
MILLER, BJ .
JOURNAL OF CLINICAL LABORATORY ANALYSIS, 1995, 9 (03) :218-224
[9]  
Harvey GR, 1999, CLIN EXP IMMUNOL, V117, P395
[10]   Specific antinuclear antibodies are associated with clinical features in systemic lupus erythematosus [J].
Hoffman, IEA ;
Peene, I ;
Meheus, L ;
Huizinga, TWJ ;
Cebecauer, L ;
Isenberg, D ;
De Bosschere, K ;
Hulstaert, F ;
Veys, EM ;
De Keyser, F .
ANNALS OF THE RHEUMATIC DISEASES, 2004, 63 (09) :1155-1158