Aqueous two-phase partitioning of xylanase produced by solid-state cultivation of Polyporus squamosus

被引：37

作者：

Antov, MG ^{[1
]}

Pericin, DM ^{[1
]}

Dasic, MG ^{[1
]}

机构：

[1] Univ Novi Sad, Fac Technol, Novi Sad 21000, Serbia Monteneg, Serbia Monteneg

来源：

PROCESS BIOCHEMISTRY | 2006年 / 41卷 / 01期

关键词：

aqueous two-phase system; partitioning; xylanase; Polyporus squamosus; solid-state cultivation; downstream processing;

D O I：

10.1016/j.procbio.2005.06.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The partitioning of xylanase, produced by solid-state cultivation of Polyporus squamosus, in aqueous two-phase system polyethylene glycol/ammonium sulphate was studied. In system 10% (w/w) polyethylene glycol 1500/20% (w/w) ammonium sulphate/70% (w/w) crude enzyme at pH 5.1 favourable partition coefficient and the top phase yield were obtained, 85.6% and 97.37%, respectively, followed by purification factor 4.8. This result suggests the possible integration of cultivation and primary downstream processing step by partitioning of xylanase in aqueous two-phase system. (C) 2005 Elsevier Ltd. All rights reserved.

引用

页码：232 / 235

页数：4

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