Cultivation of Polyporus squamosus for pectinase production in aqueous two-phase system containing sugar beet extraction waste

被引：22

作者：

Antov, MG ^{[1
]}

Pericin, DM ^{[1
]}

Dimic, GR ^{[1
]}

机构：

[1] Univ Novi Sad, Fac Technol, Dept Appl Chem, YU-21000 Novi Sad, Yugoslavia

来源：

JOURNAL OF BIOTECHNOLOGY | 2001年 / 91卷 / 01期

关键词：

aqueous two-phase system; pectinases; Polyporus squamosus; sugar beet extraction waste;

D O I：

10.1016/S0168-1656(01)00294-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Cultivation of the fungus Polyporus squamosus for pectinase production was studied in a polyethylene glycol/crude dextran aqueous two-phase system, with sugar beet extraction waste as pectin source. Fungal growth was restricted to the bottom phase and the amounts of biomass and exo-pectinase activity produced were superior to in homogeneous cultivation. The partition coefficients of endo-pectinase and exo-pectinase were 4.26 and 2.78, respectively. The top phase yields in the single extraction step were about 90% for both pectinases. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：83 / 87

页数：5

共 16 条

[1]

CONSTITUTIVE EXO-PECTINASE PRODUCED BY ASPERGILLUS SP CH-Y-1043 ON DIFFERENT CARBON SOURCE [J].

AGUILAR, G ;

HUITRON, C .

BIOTECHNOLOGY LETTERS, 1990, 12 (09) :655-660

[2]

ALPHA-AMYLASE PRODUCTION IN AQUEOUS 2-PHASE SYSTEMS WITH BACILLUS-SUBTILIS [J].

ANDERSSON, E ;

JOHANSSON, AC ;

HAHNHAGERDAL, B .

ENZYME AND MICROBIAL TECHNOLOGY, 1985, 7 (07) :333-338

[3]

BIOCONVERSIONS IN AQUEOUS 2-PHASE SYSTEMS [J].

ANDERSSON, E ;

HAHNHAGERDAL, B .

ENZYME AND MICROBIAL TECHNOLOGY, 1990, 12 (04) :242-254

[4]

Production of pectinases by Polyporus squamosus in aqueous two-phase system [J].

Antov, MG ;

Pericin, DM .

ENZYME AND MICROBIAL TECHNOLOGY, 2001, 28 (4-5) :467-472

[5]

PECTIC SUBSTANCES OF CHERRY FRUITS [J].

BARBIER, M ;

THIBAULT, JF .

PHYTOCHEMISTRY, 1982, 21 (01) :111-115

[6]

ENHANCED PRODUCTION OF SERRATIA-MARCESCENS CHITINASE IN PEG DEXTRAN AQUEOUS 2-PHASE SYSTEMS [J].

CHEN, JP ;

LEE, MS .

ENZYME AND MICROBIAL TECHNOLOGY, 1995, 17 (11) :1021-1027

[7]

Hotha S, 1997, J CHEM TECHNOL BIOT, V69, P5, DOI 10.1002/(SICI)1097-4660(199705)69:1&lt

[8]

5::AID-JCTB661&gt

[9]

3.0.CO

[10]

2-I

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