Multipotential electrochemical detection of primer extension reactions on DNA self-assembled monolayers

被引：75

作者：

Di Giusto, DA

Wlassoff, WA

Giesebrecht, S

Gooding, JJ

King, GC ^{[1
]}

机构：

[1] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia

[2] Univ New S Wales, Sch Chem Sci, Sydney, NSW 2052, Australia

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 13期

关键词：

D O I：

10.1021/ja0319036

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Electroactive nucleoside triphosphates ("electrotides") have been incorporated into primers by DNA polymerase and detected on oligonucleotide surface-assembled monolayers. Four electrotides bearing three different electroactive moietiesferrocene, vinylferrocene, and anthraquinoneare detected in four alternative formats. Copyright © 2004 American Chemical Society.

引用

页码：4120 / 4121

页数：2

共 21 条

[1] 3′-ferrocene-labeled oligonucleotide chains end-tethered to gold electrode surfaces:: Novel model systems for exploring flexibility of short DNA using cyclic voltammetry
Anne, A
Bouchardon, A
Moiroux, J
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (05) : 1112 - 1113
[2] Synthesis of the first ferrocene-labeled dideoxynucleotide and its use for 3′-redox end-labeling of 5′-modified single-stranded oligonucleotides
Anne, A
Blanc, B
Moiroux, J
[J]. BIOCONJUGATE CHEMISTRY, 2001, 12 (03) : 396 - 405
[3] Mutation detection by electrocatalysis at DNA-modified electrodes
Boon, EM
Ceres, DM
Drummond, TG
Hill, MG
Barton, JK
[J]. NATURE BIOTECHNOLOGY, 2000, 18 (10) : 1096 - 1100
[4] Use of an electrochemically labeled nucleotide terminator for known point mutation analysis
Brazill, S
Hebert, NE
Kuhr, WG
[J]. ELECTROPHORESIS, 2003, 24 (16) : 2749 - 2757
[5] A single base extension technique for the analysis of known mutations utilizing capillary gel electrophoreisis with electrochemical detection
Brazill, SA
Kuhr, WG
[J]. ANALYTICAL CHEMISTRY, 2002, 74 (14) : 3421 - 3428
[6] Single base extension (SBE) with proofreading polymerases and phosphorothioate primers: improved fidelity in single-substrate assays
Di Giusto, D
King, GC
[J]. NUCLEIC ACIDS RESEARCH, 2003, 31 (03) : e7
[7] Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases
Gardner, AF
Jack, WE
[J]. NUCLEIC ACIDS RESEARCH, 2002, 30 (02) : 605 - 613
[8] Gooding JJ, 2002, ELECTROANAL, V14, P1149, DOI 10.1002/1521-4109(200209)14:17<1149::AID-ELAN1149>3.0.CO
[9] 2-8
[10] SEQUENCE-SPECIFIC GENE DETECTION WITH A GOLD ELECTRODE MODIFIED WITH DNA PROBES AND AN ELECTROCHEMICALLY ACTIVE DYE
HASHIMOTO, K
ITO, K
ISHIMORI, Y
[J]. ANALYTICAL CHEMISTRY, 1994, 66 (21) : 3830 - 3833

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